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KULKARNI, BALAJI S.
KRISHNAMURTHY G. H.
|Title:||MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION IN GLADIOLUS (Gladiolus hybridus Hort.) VARIETIES|
|Publisher:||K.R.C. COLLEGE OF HORTICULTURE, ARABHAVI (UNIVERSITY OF HORTICULTURAL SCIENCES, BAGALKOTT)|
|Abstract:||An investigation was carried out on morphological and molecular characterization in gladiolus (Gladiolus hybridus Hort.) varieties during 2011-2013 at department of Floriculture and Landscape Architecture, Kittur Rani Channamma College of Horticulture, Arabhavi. Analysis of variance revealed highly significant difference among the 15 varieties for all the characters studied. Narrow differences between genotypic coefficient of variation (GCV) and phenotypic coefficient of variation (PCV) were observed for all the characters except for stem girth and number of leaves. Duration of flowering and number of daughter corms per plant were highly significant and positively correlated with number of spikes per plant, suggesting the possibility of simultaneous selection for these characters. Path analysis for number of spikes per plant revealed that plant height, leaf area, days taken for spike initiation, spike length, number of florets per spike, number of daughter corms per plant, had high direct effects which indicated the possibility of increasing spike yield by selecting the varieties for these characters directly. Based on morphological traits, the varieties were grouped into two clusters and the variety which are distinct can be used for further breeding programme. Molecular characterization was carried out to know the genetic diversity using PCR based Sequence-Related Amplified Polymorphism (SRAP) markers. Among twenty five primer combinations screened, eleven primer combinations gave consistent banding patterns and were used to generate the SRAP fingerprinting. The combinations of primer Me 1 + Em 3, Me 3 + Em 1, Me 4 + Em 3 and Me 4 + Em 4 produced highest polymorphism. The similarity matrix coefficient ranged from 31 to 73 per cent suggesting a moderate to higher genetic variation within gladiolus varieties. Cluster analysis showed groupings of the varieties with their geographic region of origin. On comparing the genetic diversity as revealed by the dendrogram, it was evident that Jester Gold was identified quite distinct variety. SRAP marker provided more accurate information on genetic diversity than traditional methods due to its reproducible and targets open reading frames (ORFs).|
|Subject:||Floriculture and Landscaping|
|Theme:||MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION IN GLADIOLUS (Gladiolus hybridus Hort.) VARIETIES|
|Research Problem:||MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION IN GLADIOLUS (Gladiolus hybridus Hort.) VARIETIES|
|Appears in Collections:||Theses|
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