To evaluate the effect of two species of Trichoderma against Fusarium oxypsorum on in-vitro grown Banana plant
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Date
2017
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Birsa Agricultural University, Ranchi, Jharkhand-6
Abstract
The present work has been carried out with a view to study the interaction of two species of
Trichoderma against Fusarium oxysporum f.sp. cubense (causing fusarium wilt of banana) on
in vitro grown banana plant. Isolates of five strains of T.harzianum i.e; TH1, TH2, TH3, TH4
and TH6 and one strain of T.viride i.e; TV were studied against Fusarium oxysporum on
dual-culture experiment. Maximum inhibition of mycelial growth was recorded with
T.harzianum (TH6) and T.viride (TV). Inhibition percentage of TV for 5 days is 60.03% and
9 days is 75.94% and TH6 for 5 days is 71.70% and 9 days is 86.61%.
The plants were treated for 3 and 5 days with the isolates of both the selected strain of
Trichoderma under in vitro condition. Phytochemical analysis of different parameters and
protein quantification (Bradford’s assay) of treated plants were done. Evaluation of four
different phytochemical parameter , Chlorophyll content increases upto 50- 60%,
Anthocyanin content upto20-40 %, Total soluble sugar content4-60% proline decreases upto
40-50% and protein quantification was estimated based on absorption spectrophotometry.
Description
To evaluate the effect of two species of Trichoderma against Fusarium oxypsorum on in-vitro grown Banana plant
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