IN VITRO SHOOT TIP GRAFTING IN CITRUS FOR QUALITY SEEDLING PRODUCTION.
Loading...
Date
2017-10-10
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra.
Abstract
The present investigation entitled “In Vitro Shoot tip grafting in citrus for quality seedling production” was conducted at Centre of Excellence In Plant Biotechnology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Dist.Akola (M.S.) during the academic year 2015-2017 with the objectives to study the effect of different parameters influencing shoot tip grafting efficiency and efficacy.
The experimental material of present investigation comprised the three varieties of citrus viz., Rangpur lime, Jambhiri as rootstocks and Sweet orange as scion.
For micropropagation of Sweet orange field collected nodal explants and epycotyle segments from in vitro grown seedlings was used. The multiples obtained were then used for grafting purpose on Jambhiri and Rangpur lime.
When seeds of Jambhiri, Rangpur lime and Sweet orange were inoculated for germination on various media combinations, The highest % of germination was found on MS media suplimented with 6% sucrose along with GA3 @ 1mg/l. The highest percent germination in case of Jambhiri and Sweet orange was 86.66% and in case of Rangpur the precent germination was 80.33%.
For the preparation of scion, micropropagation of Sweet orange explants viz. nodal explants from field and epicotyls segment from in vitro germinated seedlings were tried on various concentrations of BAP @ 1, 2, 3 mg/l and NAA @ 0.5 and 1 mg/l alone or in combinations. In case of field collected nodal explants the highest success percentage was 66.66% in 6 days where as vitro derived explants respond best on media combination MS + BAP @ 3mg/l in 6.33 days.
In order to optimize shoot tip grafting protocol various parameters such as sucrose concentration, age of rootstocks effect of PGRs, size of scion etc. were standardized. Amongst which sucrose % from 1.5 to 6% were tried, out of which 6% sucrose showed highest number of successful grafts on both the rootstocks Rangpur lime and Jambhiri. The rootstock age was tried from 5 to 25 days after germination and highest % success 71.42% and 62.50% in Jambhiri and Rangpur respectively was recorded when 15 days old rootstocks was used.
Different PGRs viz. kinetin (1 mg/l and 5 mg/l) and BAP (1mg/l and 5mg/l) were tried in order to enhance graft union and scion growth, the highest % success of graft was noted when scion were treated with BAP @ 1mg/l prior to graft it on rootstock either Rangpur lime (66.66%) Jambhiri (73.33%). The scion size tried varies from 0.2 to 0.7 cm amongst which the highest % of success was shown by 0.6 to 0.7 mm scions. In case of Rangpur lime the % success of graft was 58.33% where as 8.033% success was recorded in Jambhiri rootstocks.
Description
Shoot tip grafting provides opportunities in order to produce disease and virus free plantlets, to standardize explant type (rootstock and scion) and media combinations amenable for in vitro shoot tip grafting in citrus.
Keywords
null
Citation
MALKOTE, MANIK MADHAVRAO. (2017). In vitro shoot tip grafting in citrus for quality seedling production. Department of Agricultural Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. M. Sc. 2017. Print. xiii, 98p, (Unpublished).