STUDIES ON POST INSEMINATION SUPPLEMENTATION OF GnRH or hCG TO OVSYNCH PROTOCOL IN CROSSBRED COWS
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Date
2018-04-02
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PVNR TVU
Abstract
The present investigation entitled “Studies on Post Insemination Supplementation of GnRH
or hCG to Ovsynch Protocol In Crossbreed Cows” was undertaken on 50 post partum crossbreed cows to study the estrus pattern, estrus response and the efficacy of post AI supplementation of GnRH and hCG on conception rate. The 50 crossbreed cows were divided randomly into five equal therapeutic groups so that each group consist 10 crossbreed cows. The Ovsynch Group cows were injected with 10μg of GnRH(Buserelin acetate) IM on day 0, 500 μg of cloprostanol (PGF2α) IM on day 7 and 2nd dose of GnRH10μg IM on day 9. Fixed time AI was done after 16 hours of the 2nd dose GnRH of injection.Ovsynch+GnRH Group cows were injected with 10μg of GnRH IM on day 0, 500 μg of cloprostanol (PGF2α) IM on day 7 and 2nd dose of GnRH10μg IM on day 9.
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Fixed time AI was done after 16 hours of the 2nd dose GnRH of injection.On day 6 of post insemination,GnRH@ 10μg IM was administered. Ovsynch+hCG Group cows were injected with 10μg of GnRHIM on day 0, 500 μg of cloprostanol(PGF2α) IM on day 7 and
2nd dose of GnRH10μg IM on day 9. Fixed time AI was done after 16 hours of the 2nd dose
of GnRH injection. On day 6 of post insemination hCG@1500IU IM was administered. GPH+GnRH Group cows were injected with 10μg of GnRH(Buserelin acetate) IM on day 0, 500 μg of cloprostanol(PGF2α) IM on day 7 and hCG @1500IU,IM on day 9. Fixed
time AI was done after 16 hours of hCG injection. On day 6 of post insemination
GnRH@10μg IM was administered. Control Group cows were injected with 2.5 ml of NS
IM on day 0, 7 and 9 and whenever the crossbreed cows exhibited estrus then cows were
inseminated. On day 6 of post insemination 2.5 ml of NS IM was administered.
The estrus response was 90.00,100.00,100.00,100.00 and 30.00 per cent in Ovsynch, Ovsynch+GnRH, Ovsynch+hCG, GPH+GnRH and control groups, respectively. There was no significant difference (p<0.05) among the groups. The mean onset of estrus was recorded as 51.80±1.07, 50.90±1.20, 52.40 ±1.32, 52.40 ±1.32 and 96.00 ±13.86hrs in Ovsynch, Ovsynch+GnRH, Ovsynch+hCG, GPH+GnRH and control groups, respectively. The onset of estrus in therapeutic groups were not significantly (p< 0.05) different among
the groups. Among 50 post partum crossbred cows, normal intensity of estrus was exhibited by 66.00 per cent of crossbred cows whereas weak and intense estrus was
exhibited by 26.00 and 8.00 per cent of crossbred cows. The mean score for estrus intensity was recorded as 11.70±0.79, 12.50±0.83, 11.60±0.53, 12.30±0.77and 05.10±2.07, in Ovsynch, Ovsynch+GnRH, Ovsynch+hCG, GPH+GnRH and control groups, respectively. Statistical analysis revealed that the intensity of estrus in treatment groups were significantly (p<0.05) different with that of the control group. The mean duration of estrus was recorded as 21.90±0.69, 22.30±0.79, 21.80±0.73, 23.60±0.82 and 21.40±0.60 hrs in Ovsynch, Ovsynch+GnRH, Ovsynch+hCG, GPH+GnRH and control groups, respectively in the present study. The duration of estrus in theraputic groups was not significantly different among the groups.
The overall conception rate in therapeutic crossbred cows was recorded as 60.00,
50.00, 40.00, 60.00, and 20.00 per cent in Ovsynch, Ovsynch+GnRH, Ovsynch+hCG, GPH+GnRH and control groups, respectively. Statistical analysis revealed that the
conception rates were higher in Ovsynch group, GPH+GnRH group and followed by group Ovsynch+hCG, Ovsynch+GnRH and control. However, the conception rates were significantly (P<0.01) lower in control group of crossbred cows than the treatment groups.
The number of services required per conception was 1.83, 2.40, 2.75, 1.83 and 2.00 in Ovsynch, Ovsynch+GnRH, Ovsynch+hCG, GPH+GnRH and control groups, respectively. In conclusion, the present study revealed that the use of GnRH or hCG 6 days post insemination showed no significant improvement on the conception rate in crossbred cows over Ovsynch protocol.
Description
D-572
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