MARKER ASSISTED BREEDING AND GENE ACTION STUDIES IN FRENCH BEAN (Phaseolus vulgaris L.)
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Date
2013-08
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K.R.C. COLLEGE OF HORTICULTURE, ARABHAVI (UNIVERSITY OF HORTICULTURAL SCIENCES, BAGALKOT)
Abstract
French bean (Phaseolus vulgaris L.) is an important legume vegetable. It is
grown for its tender pods either for fresh consumption or for processing as canned and
freez dried products. Stringlessness is a temperature dependent trait. Beans that are
stringless at cool temperatures form strings at high temperatures due to the modifier
genes. Hence, this definitive molecular marker study was undertaken to mitigate the
occurrence of stringed and stringless individuals. Ring Beans, IC 280007 and Gokak
Local, EC 024948 genotypes were used as stringless and stringed parents respectively.
F2 populations were developed for identification of linked markers.
Eight polymorphic markers (B4, B6, B14, B16, D16, J13, L9 and I10) were
identified for stringless and stringed parents from a group of 144 SRAP primers. Primer
L9 (Me12, Em9) produced hybrid specific band of 500bp and B4 (Me2, Em4) produced
hybrid specific band of 300 bp size which might be linked with our trait of interest in
the population Gokak Local x Ring Beans and EC 024948 x IC 280007 respectively.
RAPD and ISSR markers failed to produce any amplification. Since French bean
is being a highly self pollinated crop, it will inherit all those genes which are
present in the F1 individual. Hence having gene/s controlling stringlessness in F2
individuals is quite possible.
In the cross Gokak Local x Ring Beans mode of gene action study was also
estimated through five parameter-five generation mean analysis. Predominance of
dominance gene effects were found for most of the characters where as epistatic
component was expressed for number of seeds per pod. Among two segregating
population, most of the characters had superior segregants in F2 population.
Recurrent selections like, diallel selective mating or biparental mating in early
segregating generation and selection followed by hybridization might prove to be
alternative approaches to improve the traits.
Description
TH458
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