CHARACTCRIZATION OF PEA GERM PLASM USING EST -SSR MARKERS AND BIOCLTEMICAL TRAITS
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Date
2017
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Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu
Abstract
Information about genetic diversity of any crop is Important for successful employment of breeding programmes. EST-SSRs, because of their intrinsic advatages can serve as potential tool for inferring diversity among Pisum accessions. In the present study, an initiative was taken to characterize genetic diversity among 40 different Pisum genotypes, collected from various sources/locations, using EST-SSrs and biochemical traits. For molecular characterization, thirty five EST-SSRs chosen from previous studies were used, of which only 24 could be optimized for further usage. PIC value for these markers ranged from 0.500 (Pea-069) to 0.095 (Pea-097) with an average of 0.349. Similarly, other informative parameters viz., marker index (M1) and resolving power (Rp) were also worked out. Genetic similarrity estimates based on the binomial data using Jaccard's coefficient ranged from 0.17 (Azad Pea 1/IC-219010) to 0.92 (IC-219010/219008) with an average value of 0.45 exhibiting considerable diversity among the pea genotypes studied suggesting the further use of these diverse parents in hybridization for generating transgressive segregates. The similarity estimates then used to execute clusters A and B which were further divided into sub clusters. For biochemical analysis data on three important traits viz., protein content (%), total sugar content (%) and ascorbic acid content (mg/100g) were recorded. The mean data of various genotypes for protein content (%), total sugar content (%) and ascorbic acid content (mg/100g) ranged from 25.09(IC-208366) to 16.07 (Azad P3), 19.97 (Lincoln) to 12.65 (IC-218991) and 5.57 (DPPIV-2) to 3.01 (Arka Pramodh), respectively. Identified promising lines for various biochemical traits can in future be employed to improve the target traits. Thus, information obtained from EST-SSR markers and biochemical parameters may well assist pea breeders in executing breeding programmes. Informative SSR markers exhibiting high PIC, Rp and MI can supplement in assessing genetic variability and MAS programmes. The highly divergent lines so observed can further be used in pea pre-breeding programmes to broaden the narrow genetic base of existing varieties as an assurance against unforeseen biotic and abiotic stresses.
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