EFFECT OF ANTIOXIDANT ASCORBIC ACID ON IN VITRO MATURATION OF CAPRINE OOCYTES UNDER NORMAL AND ELEVATED TEMPERATURES
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Date
2017
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Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu
Abstract
The aim of this work was to study the effect of ascorbic acid during in vitro maturation (IVM) of caprine oocytes under normal temperature (38.5° C) and heat stress condition (41° C). Influence of ascorbic acid on oocyte maturation was studied on the basis of cumulus expansion and nuclear maturation stage, Metaphase-II (MII). A total of 360 slaughter house ovaries were collected with a recovery rate of 47.20% culture grade COCs, representing 1.17 COCs per ovary. Ascorbic acid (100 µM) was added with oocyte maturation media (OMM). Without heat stress (38.5° C), ascorbic acid increased the oocyte maturation rates. Heat stress was found to lower cumulus expansion (P≤0.01) and nuclear maturation (P≤0.05). Ascorbic acid improved the maturation performance of oocytes and protected them from harmful effects of heat stress. Antioxidant ascorbic acid may neutralize heat stress generated ROS and improved cumulus expansion and nuclear maturation of oocytes.
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