DEVELOPMENTAL POTENCY OF IMMATURE OOCYTES HARVESTED FROM CRYOPRESERVED SHEEP OVARY
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Date
2017
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Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu
Abstract
The cryopreservation of reproductive cells and tissues is a growing field of research and has many applications in biomedical and reproductive research. It allows to preserve the germ plasm of high yielders, old aged animals with reproductive inefficiency, valuable animals suffering from diseases and the animal species on the verge of extinction. Cryopreservation of ovary containing immature follicle is an innovative way of preservation of female gametes. In the present study, an attempt was made to study the developmental potency of immature oocytes harvested from cryopreserved ovaries. The sheep ovaries were obtained from local slaughter house of Jammu and randomly divided into two groups i.e. fresh and cryopreserved ovaries. A total of 114 ovaries were cryopreserved for one month in liquid nitrogen (LN₂) using 10% dimethyl sulfoxide (DMSO) as a cryoprotectant. After cryopreservation, the number and nature of oocytes retrieved, histological as well as viability of follicular cells and maturation competence of the harvested oocytes were assessed and compared with those of fresh ovaries. No significant differences were observed in oocyte retrieval rates, histology of ovarian tissue and maturation rate of oocytes harvested from fresh and cryopreserved ovaries. However, only 39.76% of the retrieved oocytes from cryopreserved ovaries were able to undergone in vitro maturation (IVM) successfully. The findings of the study may provide helpful tool for further investigation aiming at cryopreservation of ovaries and assisted reproductive technique.
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