EFFECT OF SEASON ON BIOCHEMICAL PROFILE AND OXIDATIVE STRESS MARKERS IN PREOVULATORY FOLLICULAR FLUID IN LOCAL SHEEP OF JAMMU
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Date
2016
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Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu
Abstract
The investigation was undertaken in ovaries of local sheep of Jammu region to study the effect of season (autumn, winter and summer) on concentration of biochemical metabolites, minerals and oxidative stress markers in follicular fluid (FF) of preovulatory follicles. Sheep ovaries were procured from municipal slaughter house. The diameter of surface follicles was measured and grouped into small (< 6 mm), large (> 6 mm) and pooled (both small & large). From these follicles the follicular fluid was aspirated and after processing, the concentration of biochemical metabolites (glucose, total protein, albumin, globulin, total cholesterol, LDLcholesterol and nitric oxide), minerals (Fe & Mg), and oxidative stress markers (SOD & TAA) were estimated by spectro-photometric method. But the concentrations of Cu & Zn were estimated using atomic absorption spectrophotometer. It was found that season had significant (P < 0.05) effect on concentration of glucose, total protein, total cholesterol, LDL-cholesterol, copper, zinc, SOD activity and TAA in follicular fluid harvested from small and large follicles. In addition to above parameters, season had also significant (P < 0.05) effect on nitric oxide content in follicular fluid harvested from pooled follicles, but no significant difference observed in NO content of FF of either large & small follicles during different seasons. It was also observed that studied biochemical profile, mineral content of small & large follicle differed significantly (P < 0.05) in all the three seasons. The observed alterations in biochemical composition of FF during different seasons might be controlling the folliculogenesis & oogenesis process in the breed.
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