MOLECULAR CHARACTERIZATION OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) ISOLATES
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Date
2017
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Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu
Abstract
The spread of mecA gene among Staphylococcus aureus make them resistant to most antibiotics clinically in use. mecA gene can be acquired from hospitals, community as well as livestock. Therefore the present study was undertaken to characterize the methicillin resistant staphylococcus aureus (MSRA) isolates from domestic animals of Jammu region. Two hundred and twenty eight (228) samples obtained from domestic animals comprised of eighty eight (88) samples from mastitic milk, seventy seven (77) from wound pus of equine and sixty three (63) from nasal swabs of canines. Confirmation of isolates was done by polymerase chain reaction (PCR) using nuc gene specific primers for staphylococcus aureus. Out of 228 samples 51 (37.22%) isolates of S. aureus were recovered with occurrence of 21 (40.33%), 17 (41.46%) and 13 (29.54%) in cattle, equine and canine, respectively. Out of 51 isolates 8 (15.68%) yielded mecA gene specific amplicon and thus were genotypically confirmed as MRSA, while rest were MSSA. The virulence gene profile of MRSA and MSSA did not show any significant difference. Among MSSA, 86.27 % of isolates carried coa and spa gene, while 92.15% and 62.74% carried clfA and hla gene, respectively. Similarly all MRSA isolates carried coo, spa and clfA gene, while 75% isolates carried hla gene. None of the MSSA or MRSA isolates revealed the presence of lukS-PV and lukF-PV or seo gene. All the MRSA isolates were also screened for the LA-MRSA clonal-complex type CC-398 using SAPIG2194 or SAPIG2195 gene; out of these only one isolate from milk was positive for CC-398. Multiplex PCR was done for detection of other clinically relevant antibiotics. All MRSA isolates were resistant to gentamicin and oxytetracycline. Quinupristin-dalfopistrin resistance was also seen in one MRSA isolate from canine.
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