EVALUATION AND CHARACTERIZATION OF NEW IN-VITRO MUTANTS/SOMACLONES OF BANANA cv. RAJAPURI (AAB)
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Date
2016-07
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K.R.C. COLLEGE OF HORTICULTURE COLLEGE OF ARABHAVI (UNIVERSITY OF HORTICULTURAL SCIENCES, BAGALKOT)
Abstract
Evaluation and characterization of in-vitro mutants/somaclones of Banana cv. Rajapuri (AAB) was carried out in the Department of Biotechnology and Crop Improvement, KRCCH, Arabhavi during 2012-2015. Suckers having higher bunch weight (>15kg/bunch) were collected from Awargol village (Hukkeri taluk), Sangankeri village (Gokak taluk), Arabhavi village (Gokak taluk) and mutant plants resulted from in-vitro mutagenesis through irradiation treatment (25 Gy, 45Gy) from earlier study were used for the study.
The vegetative parameters like plant height, pseudo stem girth, number of functional leaves and leaf area were recorded. The treatment T10 (Somaclone Arabhavi 2) recorded the maximum plant height during the initial period of the growth up to 10 MAP. Similar trend was noticed for other vegetative growth parameters such as pseudostem girth, number of functional leaves and leaf area.
The minimum days for shooting were recorded in treatment T10 (Somaclone Arabhavi 2). The similar trend was noticed in crop duration in treatment T10 (Somaclone Arabhavi 2) which documented least crop duration. The highest bunch length, bunch width, number of hands per bunch, number of fingers on third hand and total number of fingers per bunch was recorded maximum in T10 (Somaclone Arabhavi 2). The yield characters such as bunch weight and yield per hectare was found to be highest in the treatment T10 (Somaclone Arabhavi 2).
The lowest sigatoka leaf spot index (30.81%) was recorded in the treatment of T10 (Somaclone Arabhavi 2). The genetic variations studied through Random Amplified Polymorphic DNA (primer OPC-02 and OPC-08) analysis revealed density polymorphism between plants of T10 (Somaclone Arabhavi 2), T1 (suckers) and T2 (Tissue culture plants). Hence, concluding that Somaclone Arabhavi 2 is genetically different from suckers and tissue culture plants.
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TH555
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