EFFECT OF MIXED CULTURE LIQUID FORMULATIONS (MCLF) OF BIOFERTILIZERS ON BIOCHEMICAL CONSTITUENTS IN CHICKPEA {Cicer arietenum L.)
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Date
1999
Authors
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Mahatma Phule Krishi Vidyapeeth, Rahuri.
Abstract
A pot culture experiment was conducted to study the
effect of biofertilizers on nutrient (N and P) uptake, leghaemoglobin
content, chlorophyll content, available P in the soil and activities of
nitrate reductase (NR) and acid/alkaline phosphatase. Mixed culture
liquid formulation (MCLF) of biofertilizers vizv Bioplin and
Vitromone (culture of Azotobacter spp.) and Phosfert (culture of
Bacillus spp.) along with pure culture of Azotobacter and P-solubilizer
were used for the study. Bioplin, Azotobacter and PSB were used for
seed inoculation, Phosfert was applied through soil and Vitromone
was applied as a foliar spray. Dual inoculation (seed treatment with Bioplin + soil application with Phosfert) was also used to see the
synergistic effect of Azotobacter and Bacillus culture.
Significant increase in the N and P content of the dried
leaves was noticed due to inoculation with different biofertilizers
used. Inoculation with Azotobacter culture was found to be more
effective as it helped to retain more nitrogen even upto flowering
stage. Dual inoculation (seed application with Bioplin + soil
application with Phosfert) recorded maximum N content.Seed
application with PSB and soil application with Phosfert increased the
P content by 47 per cent over uninoculated control. However, soil
application with P-solubilizer was found to be more effective over
seed inoculation. All the biofertilizers used significantly increased the
available P content in the soil rhizosphere except foliar application
with Vitromone. Seed application with P solubilizer was found to be
more effective over soil application as regard to the available P in the
soil. Seed and soil application with biofertilizers showed visual
differences in the foliage colour and this was due to significant increase in the chlorophyll va', "b' and total chlorophyll content.
Leghaemoglobin content of the root nodule tissue (expressed as
hemine equivalent) was significantly increased and seed treatment
with Bioplin recorded maximum leghaemoglobin content of 1.779
mg/g fr.wt. followed by Azotobacter 1.745 mg/g fr. wt. The activity of nitrate reductase (EC 1.6.6.1) in the leaves
was significantly increased and it was much higher at 30 DAS as
compared to flowering stage. P solubilizer in general recorded
maximum in vivo NR activity, however seed treatment was found to
be more effective. The solubilization of P might have helped in
synthesis of energy rich compound which subsequently are utilized
for energy intensive process^ nitrate assimilation. Dual inoculation
with Bioplin and Phosfert did not show any additional benefit.
Seed/soil application with P solubilizer significantly improved leaf
acid/alkaline phosphatase activity. Seed treatment with BiopHn
along with soil application of Phosfert recorded maximum acid and
alkaline phosphatase activity. The observed increase in the
phosphatase activity could help to explain the increase in the P
content in the leaves. The estimation of acid and alkaline
phosphatase activity from the root rhizosphere soil could have been
more appropriate to substantiate the results.
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