Comparative performance of rhizobial isolates from diverse soils of Uttarakhand on nodulation, growth and yield of urdbean and soil properties
Loading...
Date
2017-06
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
Eighteen urdbean (Vigna mungo L.) rhizobia were isolated from Uttarakhand soils of varying altitude and soil properties. Out of these, nine isolates showed phosphate solubilization, seven produced IAA while eleven isolates indicated siderophore production in in vitro condition. All the isolates survived well at 28˚C while, only eleven and six isolates grew at 35 and 40˚C, respectively in in vitro condition. The performance of the isolates in urdbean was compared in field study during Kharif season of 2016 at Norman E. Borlaug Crop Research Centre of the University, Pantnagar. Twenty treatments consisting of eighteen rhizobial isolates and one each of application of 20 kg N ha-1 and uninoculated control were tested in RBD in 3 replications. The test urdbean variety was Pant U-31.
Inoculation of different rhizobial isolates increased the number and dry weight of root nodules numerically, by12.0 to 39.4 and 11.6 to 29.7 % at 30 DAS and significantly, by 18.1 to 45.2 % and 12.2 to 45.5 %, at 45 DAS, respectively. The inoculated rhizobial isolates gave significant increases in plant dry weight of 25.0 to 70.8 % and 24.8 to 71.4 % at 30 DAS and 45 DAS over the uninoculated control, respectively. Grain and straw yields due to different treatments showed non-significant increases of 10.2 to 28.2 and 0.6 to 15.2 %, respectively. These treatments also significantly favoured the N content in grain, by 5.4 to 45.9 %, straw N content by 10.3 to 79.4 %, grain N uptake by 16.5 to 86.9 % and straw N uptake by 13.5 to 108.7 % over the uninoculated control, respectively. Different inoculated treatments showed numerical increase in grain and straw P content ranging from 5.4 to 35.1 % and 6.9 to 55.8 % and P uptake ranging from 20.2 to 72.4 % and 15.0 to 87.2 % over the uninoculated control, respectively. Inoculation with different rhizobial isolates significantly improved the soil microbial biomass carbon from 32.5 to 255.3 %, Organic carbon 5.1 to 96.6 %, available N from 13.3 to 71.8 % and available P from 6.1 to 88.6 % over the uninoculated control after crop harvesting. Isolate A-7 was found most efficient. The other promising isolates were P-7, A-3 and C-1 for their performance on nodulation, growth and yield of urdbean and soil properties.