CARBON MONOXIDE SENSITIVITY OF CYTOCHROME C OXIDASE AND GlBBERELLIC ACID SENSITIVITY OF SOME CHICKPEA AND PIGEONPEA CULTIVARS
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Date
1991
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Mahatma Phule Krishi Vidyapeeth, Rahuri.
Abstract
The present investigation was undertaken to monitor
the genetic differences in some chickpea and pigeonpea
cultivars by carbon monoxide sensitivity of cytochrome £
oxidase. These cultivars were also tested for gibberellin
sensitivity separately by the foliar application of GA3
solution to investigate the relationship existing if any
between the carbon monoxide sensitivity and GA3-sensitivity
of these crops. The seeds of chickpea and pigeonpea were
sown in black calcareous soil under natural day light.
After germination the seedlings were daily irrigated with
15 mM potassium nitráte. The leaves of 14-day. -old seedlings
were ušed to screen carbon monoxide sensitivity by measuring
nitrite production during jjx vivo nitráte reduction assay
under aerobic and anaerobic conditions. All the five cultivars of chickpea and pigeonpea were found to be completely insensitive to carbon monoxide, indLcating that the cytochrome a^ in these cultivars was largely in the oxidized statě.- The two cultivars of chickpea and one of pigeonpea cultivar were tested for their COspnsitivity at the difined CO:02 ratios. The cultivars of chickpea and pigeonpea responded to all the CO:0_ ratios trird, however, the CO response declined considerably as the ratios were decreased from 40 to 20. A higher concentration of CO was found to be necessary for the formation of cytochrome a-.-CO complex. Pretreatment with an uncoupler (DNP) of the oxidative phosphorylation prior to CO-treatment increased the CO response, indicating that the tightness of coupling of oxidative phosphorylation with the electron transport governs the CO-sensitivity. The stress treatment also increased the CO response, probably by the uncoupling effect.
The five cultivars of chickpea and pigeonpea were also tested for gibberellin-sensitivity. It was observed that all the cultivars were GA--sensitive and response to GA~ increased with an increase in the gibberellin concentration.
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