GENETIC CHARACTERIZATION OF NATIVE CHICKEN OF HIMACHAL PRADESH USING MOLECULAR MARKERS
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Date
06-07-11
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CSKHPKV, Palampur
Abstract
ABSTRACT
Sixty five (65) blood samples at random from birds procured from different
locations of the state and maintained at CSKHPKV Poultry farm, Palampur were collected. Fifteen
microsatellite markers (MCW073, MCW005, MCW004, MCW051, MCW049, MCW048,
MCW001, MCW014, ADL-158, ADL-172, ADL-267, ADL-210, ADL-171, ADL-176, ADL136)
which gave amplification were included in the analysis. The PCR products for different
microsatellite loci were resolved on 6% denaturing (urea) polyacrylamide gels along with 50 and
100 bp DNA ladders at 40-45V. Microsatellite alleles were visualized by silver staining. The
observed alleles, effective number of alleles, gene frequency, observed heterozygosity, expected
heterozygosity were estimated through POPGENE software. The mean observed and effective
numbers of alleles were found to be 5.87 ± 0.28. and 4.19 ± 0.17 respectively across all loci. Total
88 alleles were observed with maximum alleles (8) contributed by locus MCW073, ADL-176 & and
the least alleles (4) by (ADL-171, ADL-136, MCW014). The average heterozygosity was observed to
be 0.76 ± 0.01 across all loci. The average observed and expected heterozygosity 0.56 ± .03 and
0.77 ± 0.01 respectively. The observed heterozygosity (0.8900) was highest for ADL-176, and least
(0.4082) for ADL-267. The Expected heterozygosity (0.8278) was highest for MCW073 and least
(0.6890) least for MCW014. All the microsatellites had shown high levels of genetic variability.The
screened loci were not found to be in accordance with Hardy-Weinberg equilibrium for several
microsatellite frequencies.
Genes ChB6 and IL-2Rγ were used to study nucleotide polymorphism related to
immunocompetence The PVU-II restriction enzyme digestion of 215bp amplicon of ChB6 gene
generated fragments of 215,147 & 68 bp and revealed highly polymorphic patterns. The Hph1
restriction enzyme digestion of 600bp amplicon of IL-2Rγ gene generated fragments of
465,454,134,104,42bp and revealed highly polymorphic patterns. Fragment pattern revealed all
the genes have high level of polymorphism in DNA samples of Native Chicken of Himachal
Pradesh.
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