F2 screening of sesamum cross by molecular markers

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Date
2014-12-31
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Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani
Abstract
Sesamum (Sesamum indicum L.), a member of the order Tubiflorae, family Pedaliaceae genus Sesamum and species Sesamum indicum, having chromosome number 2n-26. is perhaps the oldest oilseed known and used by human beings (Weiss, 1971). It is an important annual oilseed crop in the tropics and warm subtropics, where it is usually grown in small plots (Bedigian and Harlan, 1986). It is a short duration crop grown throughout the year. The seeds of the plant yield edible oil. Due to the presence of potent antioxidant, sesame seeds are known as “the seed of immortality”. Sesamum is described as the “Queen of oilseeds” because it contains high oil (38-54%), protein (18-25%), calcium, phosphorous, oxalic acid and excellent qualities of seed oil and meal (Prasad, 2002). Six phenotypical characters. 5 SSR. 12 ISSR and 15 RAPD primers were used to screen the F? population. Bulked segregant analysis procedure was used for identifying markers in specific regions of the genome. According to this method, comparison of four pooled DNA samples of individuals from a segregating population originating from a single cross was done. Within each pool, or bulk the individuals were identical for two individual traits, here the leaf pattern and leaf size respectively. The gene pools were B-Broad leaf, S-Small angular leaf, A-Alternate leaf pattern, and O-Opposite leaf pattern. In this If screening, Markers that are polymorphic between the pools and are genetically linked to loci determining the trait were used to construct the pools. Here out of the total 33 primers (SSR, ISSR, RAPD) screened, we found a single RAPD G-10 primer, which distinctly shows the difference between the bulks. The present study may be useful for detection of QTLs of highly diverse and vigorous genotypes for improvement of some major morphological, agronomical and quantitative traits.
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