Assessment of genetic relationship among Fusarium SPP. derived from pigeon pea (Cajanus Cajan (L) Millsp.)
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Date
2014-10-04
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Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani
Abstract
Pigeon pea is an important grain legume. The Fusarium wilt is an important soil
home disease of pigeon pea. Presently, the information on the genetic variability among
Fusarium species of pigeon pea ami their phylogenetic relationship is lacking. Therefore,
an attempt has been made and assessed nuclear rDNA based genetic variability and
phylogenetic relationship among Fusarium species of pigeon pea. 23 isolates of
Fusarium species were, collected, purified and identified. The identification report
revealed, 4 isolates belongs to species F. udum, 10 isolates belongs to F. solani, 4 isolates
of F. moniliforme and 5 isolates were of species F. semitectum. The nuclear rDNA was
amplified by using universal primer ITS1 and ITS4 and restricted with seven hexa cutter.
Among which, EcdRl and HaeUl enabled to cut nuclear rDNA of Fusarium species also
successfully in unraveled inter as well as intra-species variability of 4 species of
Fusarium associated with pigeon pea but, failed to distinguish isolates of F. solani and F.
udum from each other.
The dendrogram analysis based upon ribotyping data revealed three distinct
clusters. The genetic variability among the species ranged between 0 to 70 %. While,
genetic similarity was found in the range between 30 to 100 %. The members of species
F. solani and F. udum were found more close to each other (93.20 % genetic similarity).
However, the species F. moniliforme was also found genetically close to species F. solani
and F. udum by showing 54.6 % to 88 % genetic similarity. Further variability among
Fusarium species was also reported by sequence length polymorphism and sequence
analysis of rDNA. The rDNA sequence of species F. solani, F. udum, F. moniliforme and
F. semitectum were found 528, 549, 521 and 550 bp respectively. The phylogenetic
relationship was established by using Neighbour joining method and Clustal omega tool
revealed the close association of members of species F. solani and F. udum. It also
revealed that, 4 species of Fusarium associated with pigeon pea would have F. solani as
common evolutionary root
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