The present investigation was carried out to study the physiological variations in early and timely sown chilli (Capsicum annuum L.). Eight genotypes (IS-261, IS-262, IS-267, ML-342, PC-408, PL-412, Sel-468 and US-501) along with two check varieties Punjab Sindhuri and Punjab Tej were grown in completely randomized block design with three replication during 2016-17 for both early and timely sown crop. During early sown crop the observations on morpho-physiological, biochemical parameters and yield attributes were recorded between 100 to 190 DAT (days after transplanting) and for timely sown crop the observations were recorded between 40 to 130 DAT (days after transplanting). In all genotypes including check varieties, photosynthetic pigments and efficiency decreased till maturity during both early and timely sown crop. On the other hand, total phenol, total soluble sugar (in leaves and flowers), ascorbic acid content, proline content and lipid peroxidation increased with maturity during both early and timely sown crop. On the basis of our study, the performance of PL-412, IS-267, PC-408 and ML-342 was found to be at par during both early and timely sown crop. The content of total soluble sugars (in leaves and flowers) and fruit set % were observed maximum in genotype PL-412 that ultimately led to increased total fruit yield in this genotype during both early and timely sown crop.