IN VITRO STUDIES IN CHRISTMAS CACTUS [Schlumbergera truncata (Haw.) Moran.]
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Date
2017-07
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Christmas cactus [Schlumbergera truncata (Haw.) Moran.], is a highly valued
ornamental potted plant in the commercial market. The plants are propagated by seeds or
vegetatively by cuttings and grafting. Seeds are difficult to obtain, and their germination
rates are low. Traditional vegetative reproduction in vivo, on the other hand, may be less
efficient insofar as a limited number of plants that can be obtained. Pursuing in vitro
methodology of plant propagation appears promising to achieve large scale production. In
the present investigation, two different explant types from different position of the stem
formed multiple shoots on Murashige and Skoog (MS) medium having 6-
Benzylaminopurine (BAP) and Naphthalene acetic acid (NAA), BAP at 3mg/l proved to
be more effective for increased shoot proliferation and shoot growth from different
explant types. Longitudinally sliced segment of explant taken from apical position of the
stem when cultured on MS medium supplemented with 3 mg/l BAP gave maximum
shoot proliferation (5.6 mean numbers of shoots). Similarly, longitudinally sliced stem
segment of explant from mid position with 3 mg/l of BAP (2.3 mean numbers of shoots)
also gave good shoot proliferation as compared to transverse sections of stem segments
taken from different position of the stem. This study shows that the explant taken from
different position of the stem such as apical and mid portion and the kind of sections
made such as longitudinally sliced segment and transversely sliced segment, play a
significant role in achieving shoot proliferation and thus determining the success in large
scale production.
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