IMMUNOLOCALISATION OF MIDGUT ANTIGEN OF RHIPICEPHALUS HAEMAPHYSALOIDES BY INDIRECT IMMUNOPEROXIDASE TEST USING METHYLENE GREEN COUNTER STAIN
Loading...
Date
2011-03
Journal Title
Journal ISSN
Volume Title
Publisher
TANUVAS
Abstract
Immunolocalisation of midgut antigen of Rhipicephalus haemaphysaloides was
performed by indirect immunoperoxidase test using methylene green as counter stain. Semi
engorged adult female ticks were fixed in bouins fixative, embedded in paraffin wax and
sectioned. The tick sections were treated with sera from immunized animals showed positive
brown colour reaction on the entire surface of digestive cells and midgut epithelial membrane.
The methylene green was used for counter staining which clearly differentiated the midgut
from other tick tissues.
Description
Keywords
Veterinary Science