Drought induced biochemical changes in leaves and roots of drought tolerant and drought sensitive varieties of barley (Hordeum vulgare L.)
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Date
2017
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DEPARTMENT OF BIOCHEMISTRY AND BIOCHEMICAL ENGINEERING JACOB SCHOOL OF BIOTECHNOLOGY AND BIOENGINEERING SAM HIGGINBOTTOM UNIVERSITY OF AGRICULTURE, TECHNOLOGY AND SCIENCES ALLAHABAD-211007, U.P. INDIA
Abstract
Drought induced biochemical changes in leaves and roots of drought tolerant and drought sensitive varieties of barley (Hordeum vulgare L.)
Narayani Shukla and Yashodhara Verma
Abstract
Barley belongs to the family of 'Poaceae'. Development of high yield and drought resistant cultivars has been slow. This is because of the presence of large genotype environment interactions, causing inconsistency in yield under different environmental conditions and lack of specific methods for screening large numbers of genotypes for both yield potential and stress tolerance. Polyethylene glycol (PEG) has been used for osmotically induced drought stress studies in plants. The present work was designed to examine the biochemical response in six barley varieties (three tolerant and three sensitive) at different osmotic potential of PEG-6000 at different time intervals (24, 48 and 72 hrs). The present study includes the antioxidant activity in barley plants under drought stress.
The drought stress adversely affects biochemical parameters such as total chlorophyll content, carotenoids, relative water content, electrolyte leakage, total protein content and lipid peroxidation. Total chlorophyll content, carotenoids, relative water content, electrolyte leakage, total protein content and lipid peroxidation was found decreased under different time intervals of drought stress. Increased activities of antioxidants (SOD, CAT, GR, APX, POX and MDA) were recorded in barley plants at different time intervals (24, 48 and 72 hrs) of drought stress. Drought tolerant varieties (K-603, K-560 and K-125) showed maximum activities of antioxidants whereas drought sensitive (K-551, K-572 and K-287) barley varieties showed smallest amount of antioxidants. Among the drought tolerant varieties K-125 variety found best. This variety gives best results of all the physiological and
biochemical analyses. Maximum chlorophyll content, carotenoid content, relative water content, electrolyte leakage, antioxidant activities and highest protein content was found in K-125 variety of barley. In native polyacrylamide gel electrophoresis of SOD and CAT maximum activity was found in K-125 and least activity was found in K-551 barley plants.
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Ph. d. thesis
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