MICROPROPAGATION AND MOLECULAR CHARACTERIZATION OF POMEGRANATE (Punica granatum L.) cv. Bhagwa
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Date
2016-12
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University of Horticultural Sciences, Bagalkot
Abstract
In the present study, experiments were carried out to develop protocol for
micropropagation of pomegranate cv. Bhagwa, screening of existing gamma
irradiated M1 seed derived progenies against bacterial blight and their genotyping.
For microprpagation studies, two types of explants, viz. shoot tip and nodal explant
were tried. Out of these two explants, nodal explant was recorded the highest survival
rate (73.33 %) with low contamination (26.67 %) in 0.1 per cent HgCl2 treatment for
6 minute.
TheMS medium supplemented with BAP 2.00 mg/l + 0.5 mg/l NAA + 30mg/l
adenine sulphate + AgNO3 1.00 mg/l was recorded as best concentration for shoot
growth parameters like, highest shoot initiation (80.00 %), least number of days for
shoot initiation (17.60), highest mean number of shoots per explants (7.00), longest
length of shoot (7.67 cm) and more number of leaves per shoot (13.87). Half strength
MS medium supplemented with higher level of IBA (1 mg/l) recorded as superior
concentration for root growth parameters like, highest root initiation (86.7 %), least
number of days for root initiation (21.0), highest mean number of roots per explants
(5.6), longest mean length of root (4.0 cm).
Hundred and twenty existing gamma irradiated M1 seed derived progenies of
pomegranate cv. ‘Bhagwa’ were assessed for their genetic variability and for selection
against BLB resistance by using four morphological traits such as bacterial blight on
leaves (PDI), leaf length, leaf width and leaf thickness. High estimates of genotypic
coefficient of variation, phenotypic coefficient of variation, heritability and genetic
advance as per cent mean were recorded for all the selected traits. Among 120
progenies screened, 13 progenies shown moderately susceptible reaction, 7 shown
susceptible reaction and others exhibited highly susceptible reaction to BLB based on
PDI value. These 20 progenies were subjected for genotyping with four polymorphic
microsatellite markers namely PGCT050, PGCT51, pg14a and pg14b were identified
by using four check varieties (Bhagwa, Ganesh, Patna and Nana). Thirteen progenies
showed similar banding pattern with moderately susceptible check and seven
progenies showed similar banding pattern with susceptible individuals.
The differentiating SSR primers identified in the present study can be further
employed for screening of other populations for BLB resistance breeding.
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