MICROPROPAGATION STUDIES IN POMEGRANATE (Punica granatum L.) cv. ‘BHAGWA’ THROUGH AXILLARY BUD PROLIFERATION
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Date
2016-08
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University of Horticultural Sciences, Bagalkot
Abstract
Studies on micropropagation of pomegranate (Punica granatum L.) cv.
‘Bhagwa’ through axillary bud proliferation was carried out at Centre for Horticulture
Biotechnology, Directorate of Research, University of Horticultural Sciences, Bagalkot,
during 2015-16. The experimental material comprised of different type of explants, viz.
shoot tip, first nodal segment, axillary bud of first nodal segment, second nodal
segment, axillary bud of second nodal segment and double nodal segment. Among the
different explants, second nodal segment collected from current season growth of
matured tree recorded better culture establishment (34.44 %) and produced the highest
number of multiple shoots (4-5). Among HgCl2 treatments, the superior culture
establishment was recorded in 0.01 % HgCl2 for 3 minute (29.44 %) and in interaction
effect, the maximum culture establishment was noted in second nodal segment with
0.01 % HgCl2 for 3 minute (56.66%). The treatment MS B + BAP 2.00 mg/l + AgNO3
1.00 mg/l was recorded as the best concentration for shoot growth parameters like , the
highest shoot initiation (91.66 %), least number of days for shoot initiation (4.25), the
highest mean number of shoots per explants (5.50), the longest length of shoot (5.04
cm) and more number of leaves per shoot (14.08). Half strength MS B supplemented
with IBA (1 mg/l) was found to be superior for root growth parameters like, the highest
root initiation (71.17 %), least number of days for root initiation (18.63), the highest
mean number of roots per explants (2.72) and the longest mean length of root (2.07 cm).
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