Fractionation of bay leaf oil and its bioefficacy against root knot nematode Meloidogyne incognita
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Date
2017
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Punjab Agricultural University, Ludhiana
Abstract
The present investigation deals with Fractionation of bay leaf oil and its bioefficacy
against root knot nematode Meloidogyne incognita. The bay leaf essential oil was extracted
by hydrodistillation using Clevenger apparatus. GC-MS analysis showed the presence of
twenty one compounds. Eugenol, α-Pinene, isoeugenol, caryophyllene and 1,8-Cineole were
the major compounds present in bay leaf essential oil. The essential oil was fractionated into
its non-polar and polar fractions by column chromatography. α-Pinene, 1,8-Cineole and
eugenol were isolated from bay leaf oil. Campholenic aldehyde was prepared from α-pinene
and eugenol epoxide was prepared from eugenol using different reagents. The compounds
isolated and derivatized were identified using FT-IR, 1HNMR and 13CNMR spectroscopy.
The nematicidal activity of bay leaf oil, its fractions, compounds isolated and derivatized was
evaluated against Meloidogyne incognita at different concentrations viz.0.20, 0.40, 0.60, 0.80,
1.00, 1.25 and 1.50 mg ml-1 for egg hatch inhibition and juvenile mortality studies. Bay leaf
oil, polar fraction and eugenol were found to be very active against. M. incognita whereas αPinene
and non-polar fraction showed less activity
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