Synergistic effect of Azotobacter, actinomycetes and phosphate solubilising bacteria on growth and yield of wheat (Triticum aestivum L.)
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Date
2017
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Punjab Agricultural University, Ludhiana
Abstract
The present investigation was undertaken to evaluate the synergistic effect of four isolates
comprising Azotobacter, Sphingobacterium and two actinomycete isolates (Streptomyces
badius and W9) for their functionality traits. All the isolates were able to produce Indoleacetic
acid (IAA) and gibberellic acid (GA). Actinomycete isolate W9 showed maximum
IAA (98.80 µg/ml) and GA (154.6 µg/ml). Similarly all the isolates were tested for
siderophore production and phosphate solubilzation where Streptomyces badius exhibited
maximum catechol type of siderophore (268 µg/ml) and hydroxamate type of siderophore
(252.9 µg/ml). Sphingobacterium sp. isolate exhibited maximum phosphate solubilization
activity (37.8±0.9mg/100ml). All the four isolates were screened for their hydrolytic enzymes
production. Actinomycete W9 showed maximum amylolytic, chitinolytic, cellulolytic and
proteolytic activity with dissolution factor 1.53 cm, 1.58cm, 1.33cm and 1.25 cm
respectively. On the basis of the plant growth promoting traits, all the isolates were tested for
growth promotion of wheat crop under field conditions. In both the trials, plant growth
parameters like shoot and root length, fresh and dry weight of shoot and root, chlorophyll
content were found to be significantly enhanced in inoculation with 100% RDF +Azotobacter
+ W9 over the control. In first trial (2015-16), maximum grain yield was showed by T11
(100% RDF +Azotobacter+ W9) i.e. 51.88 q/ha as compared to control (T2 & T1) was 45.41
& 40.13 (q/ha). Similar trend was observed in second trial (2016-17), where also T5 (100%
RDF+ W9+ Azotobacter) showed maximum grain yield (49.5 q/ha) as compared to control
(40.3 q/ha). Hence, these actinomycete isolate W9 and Azotobacter are potential candidates
for enhancing plant growth that can help in the use of eco-friendly bio-fertilizers.
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