Optimization of Media for Micropropagation of Aloe barbadensis Mill.
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Date
2017
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Birsa Agricultural University, Ranchi, Jharkhand-6
Abstract
An efficient protocol for rapid in vitro propagation of valuable medicinal plant Aloe
barbadensis by using shoot tip as explants were done. Aloe barbadensis Mill. is likely to
become a major source of a number of medicinal products of high value in the coming
future. It is imperative to convert Aloe barbadensis from wild plant to plant suited to
meet the huge demand of the industries. Therefore, efficient and fast mechanized
production need to be established for Aloe barbadensis to address the need of the present
time. Understanding the biology of Aloe barbadensis plant and biochemistry of the sweet
glycosides a prerequisites for conversion of Aloe barbadensis to a modern crop. Since
ancient times, the plant has been an exemplary source of medicines. Ayurveda and other
Indian literature mention the use of plants in treatment of various human elements India
has about 45000 plant species and among them, several thousands have been claimed to
possess medicinal properties
While Aloe is best known today for its ability to treat burns, it has been used for treating
stomach disorders, headache, constipation, influenza and fever, colic, kidney ailments,
ringworms skin, hemorrhoids, wounds, dystrophy, blistering, sunburn, menstrual
problems, insomnia, snakebite, hair loss, meningitis and other elements. Therefore
efficient and fast mechanized production needs to be established for Aloe barbadensis to
address the need.
The present work deals with the establishment of a viable protocol for this valued
medicinal plant. Out of nine different hormonal regime tried for induction of
multiplication on shoot explants, MS supplemented with 6.0 mg/l BAP + 0.2 mg/l IAA +
100.0 mg/l AdSO4 was found best to induce maximum number of plantlets per explants.
For rooting, increase in auxin (IAA) and decrease in cytokinin (BAP) and adenine
sulphate was found effective. The in vitro grown plantlets were successfully transferred
to green house for hardening.
Description
Optimization of Media for Micropropagation of Aloe barbadensis Mill.
Keywords
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