Efficacy of Cadmium in managing Fusarium wilt of Tomato (Solanum lycopersicum L.) under in vitro condition
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Date
2017
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Publisher
Birsa Agricultural University, Ranchi, Jharkhand-6
Abstract
m, 60ppm, 80ppm, 100ppm and 120ppm) on Fusarium oxysporum
grown in vitro. Concentrations of cadmium optimized by inhibition of growth
percentage of inoculums of Fusarium oxysporum. Growth percentage inhibited in
40ppm-21.05%, 60ppm-31.57%, 80ppm-57.89%, 100ppm-100% and 120ppm- 100% for
7days treatment. And for 14 days treatment, growth percentage inhibited 100ppm-
73.68%, 120ppm-100%. Above all concentration these two concentrations (100ppm and
120ppm) were found to be effective on Fusarium. So, tomato plantlets were treated in
media sup plemented with these two concentrations of cadmium (100ppm and 120ppm)
for different time periods (100pp m and 120pm 7 days treatment,100ppm and 120ppm 7
days treatment + 7 days recovery, 100ppm and 120ppm 14 days treatment) under invitro
condition. The effect of cadmium on treated plants were studied with biochemical
analysis of anthocyanin, proline, total soluble sugar and chlorophyll content and
molecular analysis of total protein content followed by 1-D SDS-PAGE in contrast to
control.
The data obtained shows an increase in anthocyanin, proline, soluble su gar and
protein content of treated as well as recovered plant were observed. Chlorophyll content
was found to be decreased. This insignificant change in chlorophyll content may be due
to in vitro condition. Perhaps chlorophyll estimation may not be suitable for in vitro
studies.
Description
Efficacy of Cadmium in managing Fusarium wilt of Tomato (Solanum lycopersicum L.) under in vitro condition
Keywords
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