EFFECT OF MIXED CULTURE LIQUID FORMULATIONS (MCLF) OF BIOFERTILIZERS ON BIOCHEMICAL CONSTITUENTS IN CHICKPEA {Cicer arietenum L.

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Date
2000
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MPKV, UNIVERSITY LIBRARY RAHURI
Abstract
A pot culture experiment was conducted to study the effect of biofertilizers on nutrient (N and P) uptake, leghaemoglobin content, chlorophyll content, available P in the soil and activities of nitrate reductase (NR) and acid/alkaline phosphatase. Mixed culture liquid formulation (MCLF) of biofertilizers vizv Bioplin and Vitromone (culture of Azotobacter spp.) and Phosfert (culture of Bacillus spp.) along with pure culture of Azotobacter and P-solubilizer were used for the study. Bioplin, Azotobacter and PSB were used for seed inoculation, Phosfert was applied through soil and Vitromone was applied as a foliar spray. Dual inoculation (seed treatment with Bioplin + soil application with Phosfert) was also used to see the synergistic effect of Azotobacter and Bacillus culture. Significant increase in the N and P content of the dried leaves was noticed due to inoculation with different biofertilizers used. Inoculation with Azotobacter culture was found to be more effective as it helped to retain more nitrogen even upto flowering stage. Dual inoculation (seed application with Bioplin + soil application with Phosfert) recorded maximum N content.Seed application with PSB and soil application with Phosfert increased the P content by 47 per cent over uninoculated control. However, soil application with P-solubilizer was found to be more effective over seed inoculation. All the biofertilizers used significantly increased the available P content in the soil rhizosphere except foliar application with Vitromone. Seed application with P solubilizer was found to be more effective over soil application as regard to the available P in the soil. Seed and soil application with biofertilizers showed visual differences in the foliage colour and this was due to significant increase in the chlorophyll va', "b' and total chlorophyll content. Leghaemoglobin content of the root nodule tissue (expressed as hemine equivalent) was significantly increased and seed treatment with Bioplin recorded maximum leghaemoglobin content of 1.779 mg/g fr.wt. followed by Azotobacter 1.745 mg/g fr. wt The activity of nitrate reductase (EC 1.6.6.1) in the leaves was significantly increased and it was much higher at 30 DAS as compared to flowering stage. P solubilizer in general recorded maximum in vivo NR activity, however seed treatment was found to be more effective. The solubilization of P might have helped in synthesis of energy rich compound which subsequently are utilized for energy intensive process^ nitrate assimilation. Dual inoculation with Bioplin and Phosfert did not show any additional benefit. Seed/soil application with P solubilizer significantly improved leaf acid/alkaline phosphatase activity. Seed treatment with BiopHn along with soil application of Phosfert recorded maximum acid and alkaline phosphatase activity. The observed increase in the phosphatase activity could help to explain the increase in the P content in the leaves. The estimation of acid and alkaline phosphatase activity from the root rhizosphere soil could have been more appropriate to substantiate the results.
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