BIOCHEMICA L INVESTIGATION S O N MUCILAG E I N OKR A {Abelmoschus esculentus (L. ) Moench )
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Date
2001
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MPKV, UNIVERSITY LIBRARY RAHURI
Abstract
Present research work was undertaken to standardize a procedure for
extraction of mucilage from stem, cortex, fruit and leaves of okra cultivars and to
screen the edible and wild type of okra varieties for their mucilage content. The
mature plants of eleven edible/cultivated and four wild species of okra were collected
from the Post Graduate research farm, at Mahatma Phule Krishi Vidyapeeth, Rahun
Different plant parts such as stem as such, cortex, fruits and leaves were separated
and cut into the small pieces for the extraction of mucilage. Among the different
extraction treatments tested viz., extractant (water, dil. alkali), sample to solvent ratio
(1-50 to 1:200), penod of extraction (2 to 10 hrs.), and foculents used to coagulate
mucilage (ethanol, acetone or isopropanol), a maximum extraction and precipitation
of mucilage could be produced by using dil. alkali as extractant for at 1 100 (w/v) for
8 hrs. followed by precipitation with acetone for all the plant parts
The stem of Reshmi cultivar showed highest (0.93%) content of mucilage
followed by EMS-10 (0.90%) and G-2 (0.89%). Stem of IIHR-10 showed lowest
(0 42%) among the cultivated species of okra In wild species, stem of Abelmoschus
ficulens had highest content (0 83%) followed by Abelmoschus tuberculatus (0 71 %),
Abelmoschus esculentus (0 71%), Abelmoschus tetraphylus (0.68%) and
Abelmoschus moschatus (0 59%)
Cortex was the major source of mucilage in okra cultivars. Within the sj^sen
cultivable varieties of okra, P-2 gave maximum extraction (1.56%) of mucilage m the
cortex followed by Arka Anamika (1.53%) and Parbhani Kranti (1.52%), while IIHR10
recorded the lowest mucilage content (1.01%) in the cortex From wild species
Abelmoschus tuberculatus showed highest recovery of mucilage (1.46%) in the
cortex, followed by Abelmoschus ficulens (1.41%) and Abelmoschus moschatus
(1.28%) Both cultivable and wild species cortex showed higher extraction of
mucilage than the other plant parts such as stem, fruits and leaves.
Mucilage extraction was maximum from fruits of Punjab Padmmi (0.68%)
followed P-2 (0.63%) and Parbhani Kranti (0.60%). Lowest recovery of mucilage
was observed m the fruits of IIHR-10 (0.23%) and Red Bhendi (0 31%). Fruits of
wild species of okra gave mucilage content in the range of 0.36% in Abelmoschus
ficulens to 0.53% in Abelmoschus esculentus.
Leaves of Reshmi recorded maximum amount (0.16%) of mucilage followed
by Parbhani Kranti (0.14%) and Arka Anamika (0.13%) in cultivable species. Wild
species leaves showed higher level of mucilage content ranged from 0.19% in
Abelmoschus esculentus to 0.12% in Abelmoschus ficulens.
Stem, cortex, fruits and leaves from cultivable (P-2) and wild (Abelmoschus
moschatus) species of okra were dried and then used for extraction of mucilage.
Similar trend for mucilage extraction from these plant parts was observed on fresh
weight basis and dry weight basis. Cortex was the major site for accumulation of
mucilage (7 80%) followed by fruits (6.30%), stem (5.40%) and leaves (0 33%) in
cultivable species. In wild species accounts upto 5.81% mucilage in cortex, 4.22% in
stem, 4.0% in fruits and 0.39% in leaves.
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