STUDIES ON PEROXIDASES IN PEARL MILLET SEEDS
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Date
2000
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MPKV, UNIVERSITY LIBRARY RAHURI
Abstract
The present investigations were undertaken to study the total
peroxidase activity in dry seeds of promising pearl millet cultivars, two
CMS lines and some market samples differing in the seed physical
properties; to standardize the conditions for partial purification of pearl
millet seed peroxidases by gel filtration technique and to study isoenzyme
patterns of peroxidases in seeds of these pearl millet cultivars using
polyacrylamide gel electrophoresis (PAGE).
Studies on different genetically pure cultivars showed a wide
variation in total seed peroxidase activity (72.8 to 137.0 units/g/min). The
cultivars, RHRBH-9907 and RHRBH-9911 with lowest peroxidase activity
were identified as desirable types in relation to shelf-life of the meal. In
CMS systems, the peroxidase activity in dry seeds of hybrid was found to be
similar or close to the female parent indicating its dominance. In market
samples, the seed peroxidase activity was found to be lower for globular
seed types as compared to obvate or lanceolate types. The activity however
did not show any definite trend for seed colour. The seed peroxidase was
purified by about 10-fold using a Sephacryl S-100 gel filtration column. The
isoenzyme patterns for various cultivars on PAGE showed that the isoforms
with Rm values 0.59 and 0.65 are common in all types with one to three
additional isoforms of slow moving type. These differences in peroxidase
isoforms can be used as a marker to determine the genetic purity of pearl
millet cultivars. However, this needs to be confirmed by analyzing a larger
number of pearl millet cultivars.
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