Identification and characterization of pathogenesis-related proteins involved in Fusarium wilt resistance in chickpea
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Date
2003
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MPKV, UNIVERSITY LIBRARY RAHURI
Abstract
A pot culture experiment was conducted to identify the
pathogenesis-related proteins (PRs) and other biochemical markers
involved in resistance to Fusarium wilt in chickpea. Three resistant,
V ay', Vishaľ, and 'Phule G-93009' and three susceptible 'Phule G-5',
'Chafa' and 'JG-62' cultivars of chickpea were separately grown in
sterilized (control) and wilt-sick soils during the rabi season of 2002
The healthy and infected leaves, stems and roots of these six cultivars
were analyzed for various PRs and other biochemical constituents after
25 days of sowing when the visual symptoms of the disease appeared
on the susceptible cultivars grown in wilt-sick soil.
The acidic PRs were separated by native and SDS-PAGE
while the basic PRs were separated by native-PAGE. The results
obtained in the present investigation indicated that m the root portions
of the resistant cultivars, a maximum number of the PRs bands were
synthesized followed by stem portions as compared to the susceptible
cultivars Marked differences in PR bands were not observed m the
leaves of susceptible and resistant cultivars. Acidic PR bands of -25 7
kD, 23 kD, 33.5 kD and 66kD and a basic PR band of -22 kD appeared
to be synthesized in the root portions of the resistant cultivar, Vijay' In
another resistant cultivar, Vishaľ acidic PR bands of -66 kD in root
portions and of -32 kD in stem portions were found to be synthesized
The leaves, stems and roots of resistant cultivars viz, Vijay' followed by
Vishaľ had comparatively higher levels of total phenolics and
chlorogenic acid than all the susceptible cultivars However the leaves,
stems and roots of susceptible cultivars had higher levels of soluble
proteins After invasion of pathogen, the contents of phenols and
chlorogenic acid increased in resistant cultivars An increase in soluble
proteins, however was of higher magnitude m the susceptible cultivars.
Thus the synthesis of higher number of novel PR bands and
higher levels of phenols and chlorogenic acid in Vijay' is indicative of
the fact that this variety is more tolerant to the Fusanum oxyspontm
fungi followed by Vishaľ However, another resistant cultivar, 'Phule G93009'
did not show marked synthesis of PR bands and therefore may
be classified as susceptible rather than resistant as categorized earlier
based on the results obtained in the present investigation
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