Correlation of Theileria annulata infection in experimental calves with ELISA (antibody titre) and blood smear examination (percentage of parasitaemia)
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Date
1995-08
Journal Title
Journal ISSN
Volume Title
Publisher
TANUVAS
Abstract
Experimental infection of Thei/eria annu/ata in calves was set up to induce T.
annu/ata parasitaemia through blood/tick. A total of 63 sera samples from five calves
were collected priorto infection and at periodical weekly intervals after infection and sera
titres were assessed by ELISA and correlated with parasitaemia. Blood smear examina-
tion in four experimental calves indicated nil parasitaemia on the day of splenectomy and
7th day after splenectomy, when the antibody titre ranged from 1:1 O0 to 1:200., increase
in parasitaemia from 15-80 percent on 14th day when the antibodytitre ranged from 1 :4OO
to 111600; maximum parasitaemia oi 40 to 50 per cent on 21st day and the highest titre
of 1 :16O0 to 1:32OO on that day; and consequent gradual reduction of antibody titres from
85th day. Calf infected with T. annu/ate infected Hya/omma marginatum isaaci ticks
showed antibodytitre from 21 st day onwards and the titre was found to increase gradually.
Correlation of results of blood smear examination and ELISA indicated, that increased
antibody titre and the subsequent decrease is directly proportional to the increase and
decrease in parasitaemia.
Description
TNV_SNC_Y1995_19-21Aug_Pg120
Keywords
Veterinary Science