Development of microbial consortium for plant growth promotion of soybean (Glycine max (L.) Merrill)
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Date
2017
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Punjab Agricultural University, Ludhiana
Abstract
The present investigation was carried out to investigate the synergistic effect of consortium of Bradyrhizobium sp. (LSBR-3) with endophytic bacteria for improving growth, symbiotic efficiency, soil quality and yield in soybean. Compatibility of authenticated nine endophytes (Acetobacter sp., Burkholderia sp., Pseudomonas oryzihabitans, Pseudomonas aeruginosa, Paenibacillus sp., Pseudomonas fluorescence, Pseudomonas argentinensis, Bacillus aryabhattai and Stenotrophomonas maltophilia) was assessed with Bradyrhizobium sp. for development of consortium biofertilizer on the basis of multifarious PGP traits i.e. IAA, P-solubilization, siderophore, ACC deaminase and biofilm formation in soybean. In dual inoculants PGP traits were significantly more than single inoculants. Significantly high IAA production (54.18 μgml-1) was shown by Bradyrhizobium sp. + S. maltophilia whereas dual inoculant Bradyrhizobium sp. + P. fluorescence showed maximum P-solubilization (12.14 μgml-1). Highest siderophore production (2.40 cm) was shown by Bradyrhizobium sp. + Paenibacillus sp. Significantly high ACC-deaminase (OD600nm 1.8316) and biofilm production (OD600nm 1.3896) was shown by Bradyrhizobium sp. + P. oryzihabitans. Potential dual inoculant with multifunctional PGP traits were exploited for development of consortium biofertilizer under field. Maximum dry weight of shoot (15.70 and 23.53 g plant-1 at vegetative and flowering stage, respectively) and total chlorophyll content (1.28 and 1.37 mg/g fresh weight of leaves at vegetative and flowering stage, respectively) were recorded with consortium of Bradyrhizobium sp. (LSBR-3) and P. oryzihabitans as compared to Bradyrhizobium sp. alone as well as un-inoculated control. Symbiotic and soil quality parameters were significantly high viz. dry weight of nodules (184.3 mg), leghaemoglobin content (10.84 mg/g fresh weight of nodules), phosphatase activity (74.23 and 77.39 µg p-nitrophenol/ml/h alkaline and acidic phosphatase activity at flowering stage) and dehydrogenase activity (28.26 and 37.01 µg TPF/g/soil/h at vegetative and flowering stage, respectively) with Bradyrhizobium sp. + P. oryzihabitans treatment as compared to Bradyrhizobium sp. alone as well as un-inoculated control. Co-inoculation of Bradyrhizobium sp. and P. oryzihabitans significantly improved the grain yield by 4.1% over Bradyrhizobium sp. (LSBR-3) alone treatment. Therefore, it appears that native potential P. oryzihabitans can be explored as potent bio-enhancer and bio-fertilizer along with recommended Bradyrhizobium sp. (LSBR-3) in soybean under low input technology programme of sustainable agriculture.
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