ANALYSIS OF POMEGRANATE PEEL EXTRACT (PPE) FOR ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES
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Date
2014
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MPKV, UNIVERSITY LIBRARY RAHURI
Abstract
Pomegranate peels are usually discarded as waste
even though a significant portion of polyphenols are often
present in high concentration in the peel of the fruits. The
objective of the present investigation was to establish suitable
solvent(s) for extraction of phytochemicals present in the fresh
and dried pomegranate peel and to assess their potential radical
scavenging activity as well as their inhibitory potential against
some plant pathogenic bacteria and fungi. Three solvents
methanol:water (80:20), acetone:water (80:20) and ethyl acetate
were used for extracting the phytochemicals. The
methanol:water (80:20) extract recorded maximum content of
phenols and ethyl acetate was least effective. Out of the four
varieties, the fresh peel extract of Ganesh variety recorded
maximum phenol content of 66.23 mg/g when extracted with
methanol and the least phenol content of 9.04 mg/g was
recorded in Mridula with a ethyl acetate. The superiority of the
solvent was in the order methanol>acetone>ethyl acetate. The
methanol:water (80:20) was also found to be effective for
extracting proanthocyanidins and maximum proanthocyanidins
4.14mg/g and 12.88mg/g were extracted from fresh and dry
peel respectively of pomegranate Mridula variety. Both methanol
and acetone were found to be equally effective for extracting
proanthocyanidins. The content of flavonoids were also
maximum in the peel extract of Ganesh when extracted with
methanol, which was maximum in Ganesh 5.32mg/g where as it
was minimum 2.44mg/g in Jodhpuri Red. The antioxidant
activity of the dry peel extract when analysed by DPPH and
FRAP assay, it revealed that the antioxidant activity of the
acetone:water (80:20) extract was higher than the synthetic
antioxidant BHT and it was also observed that the antioxidant
activity increased in a dose dependent manner. The maximum
FRAP value of 46.15 µM/g was recorded in Ganesh and least in
Jodhpuri Red. The methanolic extract recorded more FRAP
value, when expressed as equivalent to that of 1mM FeSO4+.
The antibacterial activity of the extracts were tested by disc
diffusion method and it was observed that methanolic extract of
fresh peel showed highest zone of inhibition when tested against
Xanthomonas spp. It was also observed that only methanolic
extract showed antifungal activity against Fusarium oxysporum.
When the antifungal activity against Aspergillus flavous was
tested by methanolic extract of Ganesh at different
concentration it was observed that inhibition of growth
increased in a dose dependent manner.
The methanol:water (80:20) was found to be the best
solvent mixture for extracting the phytochemicals from
pomegranate peel and the peel extract of Ganesh variety of
pomegranate recorded maximum content of phenols,
proanthocyanidins, flavonoids and also had more antioxidant
potential.
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