BIOCHEMICAL ANALYSIS OF BT COTTON HYBRIDS IN RELATION TO SUCKING PESTS TOLERANCE

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Date
2012
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MPKV, UNIVERSITY LIBRARY RAHURI
Abstract
The Present investigation entitled “Biochemical analysis of Bt cotton hybrids for sucking pest tolerance” was mainly focused for analyzing the biochemical constituents and the level of sucking pest tolerance at different growth stages. The total polyphenol content in the leaves of Bt cotton hybrids ranged from 2.56 to 6.05 mg g-1 fr.wt. at 30 DAS. with maximum of 6.05 mg g-1 fr.wt. being present in Dyna and the minimum of 2.56 mg g-1 fr.wt. in Tulasi. At 60 DAS the content of polyphenol ranged from 4.14 mg g-1 fr.wt. to 7.91 mg g-1 fr.wt. with the maximum of 7.91 mg g-1 fr.wt. being present in NHH-44 and the minimum of 4.14 mg g-1 fr.wt. in Tulasi. with At 90 DAS the content of polyphenol ranged from 5.50mg g-1 fr.wt. to 8.41 mg g-1 fr.wt. with maximum of 8.41 mg g-1 fr.wt. in Tulasi and the minimum of 5.50 mg g-1 fr.wt. in Rasi-2. The tannin content in leaves of eight Bt cotton hybrids ranged from 1.67 to 3.98 mg g-1 fr.wt. at 30 DAS . The maximum of 3.98 mg g-1 fr.wt. being present in Dhuv and the minimum of 1.67 mg g-1 fr.wt. in NHH-44, Bt cotton hybrid and at 60 DAS ranged from 1.77 to 8.74 mg g-1 fr.wt. at 60 DAS. The maximum of 8.74 mg g-1 fr.wt. being present in Monsoon and the minimum of 1.77 mg g-1 fr.wt. in NHH-44 and at 90 DAS ranged from 3.21 to 8.35 mg g-1 fr.wt. with maximum of 8.35 mg g-1 fr.wt. being present in Monsoon and the minimum of 3.21 mg g-1 fr.wt. in NHH-44 The chitinase activity in leaves of Bt cotton hybrids ranged from 0.79 to 4.75 mg N-acetylglucosamine released g1 fr.wt.h-1 at 30 DAS. The maximum chitinase activity of 4.75 mg N-acetylglucosamine released g-1 fr.wt. h-1 being present in Dyna and the minimum of 0.79 mg N-acetylglucosamine released g-1 fr.wt. h-1 in Mallika. At 60 DAS the activity ranged from 1.34 to 4.98 mg N-acetylglucosamine released g-1 fr.wt. h-1. The maximum of 4.98 mg N-acetylglucosamine released g-1 fr.wt. h-1 being present in Rasi-2 and the minimum of 1.34 mg N-acetylglucosamine released g-1 fr.wt.h-1 in Tulasi and at 90 DAS it ranged from 2.93 to 6.65 mg N-acetylglucosamine released g-1 fr.wt. h-1 with maximum of 6.65mg N-acetylglucosamine released g-1 fr.wt. h-1 being present in Dhuv and the minimum of 2.93 mg N- acetylglucosamine released g-1 fr.wt. h-1 in Bunny. The peroxidase activity in leaves of eight Bt cotton hybrids ranged from 0.94 to 2.24 U g-1 fr. wt. at 30 DAS. The maximum of 2.24 U g-1 fr. wt. being present in Dhuv and the minimum of 0.94 U g-1 fr. wt. in Dyna. Bt cotton hybrid and at 60 DAS the activity ranged from 1.04 to 2.65 U g-1 fr. wt. The maximum of 2.65 U g-1 fr. wt. being present in Dyna and the minimum of 1.04 U g-1. fr.wt. in Dhuv. At 90 DAS peroxidase activity ranged from 2.13 to 5.00 U g-1 fr. wt.. The maximum of 5.00 U g-1 fr. wt. being present in Monsoon and the minimum of 2.04 U g-1 fr. wt. in Tulasi. The polyphenol oxidase activity in leaves of eight Bt cotton hybrids ranged from 0.85 to 2.22 U g-1 fr. wt. at 30 DAS. The maximum of 2.22 U g-1 fr. wt. being present in Dyna and the minimum of 0.85 U g-1 fr. wt. in Monsoon and at 60 DAS the PPO activity ranged from 1.03 to 2.56 U g-1 fr. wt. The maximum of 2.56 U g-1 fr. wt. being present in Dyna and the minimum of 1.03 U g-1 fr. wt. in Monsoon. At 90 DAS ranged from 1.03 to 3.22 U g-1 fr. wt. The maximum of 3.22 U g-1 fr. wt. being present in Dyna and the minimum of 1.03 U g-1 fr. wt. Dhuv which is moderately resistant against the sucking pest infestation had higher biochemical constituents at different growth stage. The sucking pest infestation is negatively correlated with total polyphenol, tannin, chitinase and peroxidase, polyphenoloxidase at different growth stages.
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