BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF GENOTYPES IN CAULIFLOWER (BRASSICA OLERACEA VAR. BOTRYTIS L.)
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Date
2016
Authors
Journal Title
Journal ISSN
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Publisher
Division of Vegetable Science ICAR-Indian Agricultural Research Institute (IARI) New Delhi-
Abstract
The present investigation entitled “Biochemical and molecular characterization of
genotypes in cauliflower (Brassica oleracea var. botrytis L.)” was undertaken at the
research farm of Division of Vegetable Science, IARI, New Delhi during 2015 to 2016.
This study was conducted to assess morphological, biochemical, and molecular diversity
in cauliflower. A total of 48 genotypes comprising early, mid-early and mid-late were
characterized for 15 morphological and five biochemical traits. Analysis of variance
(ANOVA), cluster analyses (CA), principal components analysis (PCA) elucidated
patterns of variation among genotypes. In early group, the average marketable curd
weight was 0.358 kg which was statistically different from most of the genotypes. The
genotype CC 12 had maximum marketable curd weight (0.450 kg). The average net curd
weight was 0.318 kg and maximum was observed in CC 15 (0.377 kg). In morphological
characters, highest GCV (30.89%) and PCV (41.21%) were found for marketable curd
weight. The biochemical properties such as curd sinigrin was found to be maximum in
DC 41-5 (16.37 µmol 100g
-1
FW) whereas CC 13 had 15.43 µmol 100g
-1
FW of leaf
sinigrin. Highest GCV (57.22%) and PCV (57.25%) were recorded for curd sinigrin. Net
curd weight (kg) and yield q/ha were recorded highest for Pusa Sharad with 0.310 kg and
103.33 q/ha, respectively for mid-early group. DC 326 attained highest curd sinigrin of
36.93 µmol 100g
-1
FW whereas leaf sinigrin was highest for DC 306 (39.50 µmol 100g
-1
FW). The average net curd weight of mid-late cauliflower was 0.271 kg, where CC 32
and DC 431 had highest net curd weight (0.387 kg). Curd and leaf sinigrin were detected
maximum for Pant Gobhi 2 and Selection 1-2 with 16.45, 17.56 µmol 100g
-1
FW,
respectively. Mid-early genotypes had maximum glucosinolate (sinigrin). The average
antioxidant (CUPRAC and FRAP assay) and total phenol content were highest in early
genotypes though mid-early genotypes comprised of highest ascorbic acid content.
Eigen value for each PC axis with first principal component (PC1) explained 47.72% of
the total variation among the 48 cauliflower genotypes for describing twelve traits,
followed by PC2 (24.55%) and PC3 (10.35%), merely 41.33%, 28.57% and 10.37% of
66 | P a g e
PC1, PC2 and PC3, respectively were observed in biochemical traits among 48
cauliflower genotypes. In molecular diversity stydy, 26 polymorphic primers produced
band ranging from 2.00 to 5.00. The UPGMA dendrogram allocated 48 cauliflower
genotypes into six major clusters. Hence, morphological, biochemical and molecular
markers employed in this study were effective in discriminating the 48 cauliflower
genotypes. In future, the diverse genotypes can be used as genetic stocks in breeding
programs of cauliflower.
Description
t-9492
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