WHOLE GENOME SEQUENCE CONVERSION DOWNSTREAM OF THE MinION PLATFORM USING PORETOOLS
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Date
2017-10
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Abstract
The MinION portable DNA sequencer from Oxford Nanopore Technologies (ONT) has been
released to the scientific community with in the frame work of an early access programme to
evaluate its application for a veraity of genetic approaches. Nanopore sequencing may be the
next disruptive technology in genomics, owing to its ability to detect single DNA molecules
without amplification This Technology has provide a genome wide analyses. In this study we
tested the ability of MinION system that permits real-time analysis of streaming event to perform
the Whole genome sequencing of Aedes aegypti mosquito. Poretools is a flexible toolkit for
exploring data generated by Nanopore sequencing device MinION for the purpose of
downstream analysis. In which we convert the raw data generated in a set of FAST5 file from
one sequencing run to FASTA or FASTQ format.
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