Diversity in Lentil (Lens culinaris M.) based over Morphological and Molecular Markers
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Date
2014
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MPUAT, Udaipur
Abstract
The present study entitled as “Diversity in Lentil (Lens culinaris M.) based
over Morphological and Molecular Markers” was carried out using 30 diverse
varieties/strains for seed yield and its components. The material was planted during rabi,
2013-14, at Rajasthan College of Agriculture, Udaipur and Agriculture Research Station,
Kota. Observations were recorded for 10 yield contributing characters and seed protein
content.
Variability parameters, correlation, path coefficient and genetic divergence
(mahalanobis, 1936) were computed for each location separately and over pooled basis.
DNA was isolated with CTAB extraction buffer method. DNA concentration and purity
was checked by spectrophotometer and agarose gel electrophoresis. Isolated DNA was
used as template for amplification of DNA using 20 randomly selected decamer primers.
To conclude, characters like pods/plant, biological yield/plant and 100-seed
weight had high estimates of all variability parameters and were strongly correlated with
each other having maximum direct effects towards seed yield appeared promising to
contribute genetic diversity in lentil over locations.
Based over field studies conducted over 2 locations, divergent and high yielding
genotypes in clusters VI/VII/I (RKL 603-1, IPL406, RKL 606-09, DPL-62, RKL1003-
59F, RKL1017, RKL1003-53E, RKL1003-44E and RKL1003-46E) were identified. It is
interesting to record that using RAPD analysis, above genotypes were also classified as
most divergent belonging to different clusters. Therefore RAPD analysis appeared as a
precise, rapid and an efficient technique in identifying divergent genotypes. However, for
the evaluation of per se performance multilocation field testing is essential and important.
In the light of these facts, conventional breeding methods coup
Description
Diversity in Lentil (Lens culinaris M.) based over Morphological and Molecular Markers
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Citation
Kunwar and Maloo, 2014