Character Association and DNA profiling in Groundnut (Arachis hypogaea L.)
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Date
2015
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Publisher
MPUAT, Udaipur
Abstract
The present investigation entitled “Character Association and DNA profiling
in Groundnut (Arachis hypogaea L.)” was conducted with 30 genotypes (including
three checks namely Pratap Raj Mungphali, Pratap Mungphali-2 and TG-37A)
during Kharif- 2014 at the Instructional Farm, College of Technology and
Engineering, Maharana Pratap University of Agricultural and Technology, Udaipur.
The genotypes were planted in a randomized block design with three replications.
The observations were recorded for 13 characters viz., days to 50% flowering,
days to maturity, plant height, number of branches per plant, number of matured pods
per plant, dry pod yield per plant, kernel yield per plant, 100-kernel weight,
sound mature kernel, shelling out turn, biological yield per plant, harvest index and
seed oil content on five randomly selected plants from each genotype in all the
replications while days to 50% flowering and days to maturity which were recorded
on plot basis and average value was subjected to analysis of variance, estimation of
variability parameters, correlation and path coefficient.
The estimates of genotypic parameters revealed that differences between the
estimates of GCV and PCV were found least for most of the characters. Higher
estimates of GCV were observed for number of mature pods per plant, Kernel yield
per plant and Dry pod yield per plant. Maximum heritability was found for 100 kernel
weight followed by oil content and sound mature kernels. While, maximum genetic
gain was observed for Dry pod yield per plant followed by kernel yield per plant, 100-
kernel weight and number of mature pods per plant.
Association estimate revealed that dry pod yield per plant was positively
correlated at both genotypic and phenotypic levels with kernel yield per plant,
biological yield per plant, harvest index, 100-kernel weight, sound mature kernels and
oil content. Similarly, Kernel yield per plant also showed positive correlation with dry
pod yield per plant, biological yield per plant, harvest index, number of branches per
plant, 100-kernel weight and oil content, at both genotypic and phenotypic levels. Present experimental findings revealed that characters, number of mature pods
per plant, dry pod yield per plant, kernel yield per plant and 100 kernel weight,
showed high GCV, heritability and genetic gain. Hence, selection can be made for
improvement of these characters.
Path coefficient analysis revealed maximum direct effect by kernel yield per
plant (0.91). While, other characters viz., biological yield per plant (0.61), number of
branches per plant (0.32) and 100 kernel weight (0.24) exhibited high indirect effects
on dry pod yield through kernel yield per plant.
Nine markers were used for RAPD analysis and all markers showed
amplification. A dendrogram was constructed from similarity coefficients obtained for
DNA banding pattern. The clusters obtained by dendrogram could also be
distinguished by similarity for the morphological characteristics within each group
and diversity between clusters. Such an association may used for more effective
breeding programmes
Description
Character Association and DNA profiling in
Groundnut (Arachis hypogaea L.)
Keywords
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Citation
Manlata and Sharma, 2015