Assessment of SSR Markers for Shoot Fly Resistance in Back Cross Generation of Sorghum [Sorghum bicolor (L.) Moench]
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Date
2015
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Publisher
MPUAT, Udaipur
Abstract
In the present investigation F1 was obtained between shoot fly resistant parent SUENT11 and susceptible parent 27B. F1 (27B X SUENT11) was
back crossed with 27B to obtain 27B X (27B X SUENT11) BC1 population. The parents used for the study showed variation in morphological
observation for shoot fly resistance. Back cross progenies varied from in between 27B to SUENT11. The character of F1 revealed that resistance was
dominant over susceptibility.
50 BC1 plants along with both parents were subjected to PCR analysis. Purified DNA of both the parents, F1 and BC1 plants were subjected to
PCR based SSR markers to determine shoot fly resistance. The ratio of A260/A280 was ranged from 1.8 to 1.94 which indicate that DNA was of good
quality. The concentration of DNA range between 45 ng/μl to 528 ng/μl. Out of 21 SSR primers used only 6 primer were amplifed according to SSR
pattern, but all were having monomorphic bands. The 18 BC1 plant showed high early vigour, more glossiness, less ovipositions and high trichome
density were identified for future advancement to obtain shoot fly resistant plant.
Description
Assessment of SSR Markers for Shoot Fly Resistance in Back Cross Generation of Sorghum [Sorghum bicolor (L.) Moench]
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Citation
Khan and Ranwah, 2015