RAPD Analysis in Ashwagandha [Withania somnifera (L.) Dunal]
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Date
2014
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MPUAT, Udaipur
Abstract
The present investigation entitled “RAPD analysis in Ashwagandha [Withania
somnifera (L.) Dunal]” was carried out with 20 genotypes. The genotypes were
planted in randomized block design with three replications during late kharif- 2012-
2013 at Research Farm, Department of Plant Breeding and Genetics, Rajasthan
College of Agriculture, MPUAT, Udaipur.
The observation were recorded for nine characters viz., plant height, number of
primary branches per plant, days to 75 per cent flowering, days to 75 per cent
maturity, root length, root diameter, number of primary branches per plant, dry root
yield per plant and alkaloid content on ten randomly selected plants from each
genotype under all replications for all the above characters except days to 75 per cent
flowering and days to 75 per cent maturity which were recorded on plot basis and
averaged value was subjected to analysis of variance, estimation of different
variability parameters, correlation and path coefficient analysis.
Fifteen markers were used for RAPD analysis and nine markers showed
amplification. A total 46 scorable bands were produced, out of which 41 bands were
polymorphic and the level of polymorphism was 89.13 per cent. A dendrogram was
constructed from similarity coefficients obtained for DNA banding pattern. The
groups/clusters obtained by dandrogram could also be distinguished by
dissimilarity/similarity for the morphological characteristics within each group. Such
an association may be used for more effective breeding programmes.
Analysis of variance revealed that mean square due to genotype were highly
significant for all the characters indicating considerable amount of variability for all
the characters and were amenable to improvement. Highest GCV and PCV were obtained for number of berries per plant, dry root yield per plant, number of primary
branches per plant and root diameter. In general, magnitude of PCV was higher than
the magnitude of GCV. Highest heritability estimates was recorded for number of
berries per plant followed by days to 75 per cent flowering, dry root yield per plant,
number of primary branches per plant, days to 75 per cent maturity and root diameter.
Expected genetic gain was observed highest for number of berries per plant followed
by dry root yield per plant, number of primary branches per plant and root diameter.
Selection for these characters would be effective in selection of suitable genotype for
Ashwagandha improvement.
The magnitude and direction of correlation coefficient analysis in the present
study could not explain the clear cut picture of direct association of dry root yield and
its component traits. However, it can be concluded from the correlation study that
emphasis can be laid on few of the component trait for improving the dry root yield in
Ashwagandha as some of the component traits showed positive/negative significant
association among themselves.
Path coefficient analysis revealed that the characters viz. days to 75 per cent
flowering, number of berries per plant, alkaloid content, days to 75 per cent maturity
and plant height were the important characters for dry root yield because these had
direct effect on dry root yield. These characters also had positive indirect effect with
each other. So selection for these characters would be effective in selection of suitable
genotype for Ashwagandha improvement
Description
RAPD Analysis in Ashwagandha [Withania somnifera (L.) Dunal]
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Citation
Sahu and Dodiya, 2014