Tissue Culture Studies in Jatropha (Jatropha curcas L.)
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Date
2006
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MPUAT, Udaipur
Abstract
The present study entitled "Tissue Culture Studies in Jatropha (Jatropha
curcus L.)" was completed in Biotechnology Laboratory, Department of Plant Breeding and
Genetics, Rajasthan College of Agriculture, Udaipur.
The main objectives of the study were to find out effective concentrations for shoot
and root regeneration from 2.5 to 3 cm long stem with and without apical buds.
For shoot regeneration, explants were cultured on MS medium supplemented 3.0 and
5.0 mg/l concentrations of cytokinin (BAP & Kn) and auxin (IBA & NAA) along with
control. Swelling and shooting were observed on treatment 3 and 5 mg/l Kn in explants
having apical bud. In another experiment when 2.5 to 3 cm long stem tip inoculated on MS
medium supplemented with i.e. 3, 4, 5, 7, 10 & 15 mg/l Kn in the month of April, May and
June. The April months inoculation was best season for micropropagation as in this month
days to swelling and days to shooting was earliest and number of sprouted shoots per explant
was also maximum followed by June and May. Among the treatments earliest days to
swelling and days to shooting were observed in 4 mg/l Kn. When effect of treatments were
seen in each month the treatment 10 mg/l Kn was having earliest for days to shooting in
April and May inoculation and 4 mg/l Kn was earliest in June inoculation. Whereas among
the months within treatment April inoculation took minimum days to swelling and days to
shooting in all the treatments applied in this month i.e. 4, 5, 7 & 10 mg/l Kn followed by June
inoculation and May inoculation.
On full strength MS media supplement with 5 mg/l IBA + 3 mg/l NAA instead of
rooting on sprouted shoots callusing was there, whereas when this concentration was applied
on half strength MS media and shoots were treated with 500 mg/l IBA solution for 10 minutes
before inoculation neither rooting nor callusing was observed and at least shoots become
dried.
Description
Tissue Culture Studies in Jatropha (Jatropha
curcas L.)
Keywords
null
Citation
Chopra and Ranwah, 2006