RICE DOUBLED HAPLOID PRODUCTION AND HOMOZYGOSITY TESTING USING PHENOTYPIC AND MOLECULAR APPROACHES
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Date
2017
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Indira Gandhi Krishi Vishwavidhyalaya, Raipur
Abstract
Anther culture based double haploid (DH) production is a technology which, can significantly reduce the time period require for development of new crop variety. In the present investigation, on attempt is made to develop DHs using anther culture in rice (Oryza sativa L.). two crosses Swarna sub 1 x IR 90019-17-159-B and MTU1010 x Dagaddeshi were subject for the study. The anther are excised and plated on to N6 (Chu. 1978) media supplemented with 3% maltose 0.8% agar and the pH was maintained of 5.8 and 2 ml/l 2,4-D. In cross Swarna sub 1 x IR 90019-17-159-B highest number of callus was induce 23.44% and cross MTU1010 x Dagaddeshi 12.19%.The induce callus was transfer to 2 different media T11, T15 along with control for regeneration.T15 was found to be best as it has produce 254 number of green plant were in Swarna sub 1 x IR 90019-17-159-B and 5 number of green plant MTU1010 x Dagaddeshi.(Rukminiet al. 2013)
Morphological charactrization and molecular markers were used to differentiate between diploid and DHs plants. DUS assay and SSR marker are used for differentiate DHs. The line selected are DHs line of kharif 2015 developed through anther culture at PMBB, Raipur . 60 plants randomly selected from all 6 DH lines S-17 x RYT-3275(185) S-17 x PB(717), S-17 x IR-64(78), S-17 x IR-64(600), S-17 x IR-64(114), S17 x RP-BIO(1246). All the line are individually assessed for their genetic stability and homozygosity using SSR markers. In DUS assay character like plant height , Panicle length, Number of tiller , Number of panicle, Flag leaf length ,Flag leaf width, Grain length, Grain width, 1000 seed weight , Seed weight per/plant has showen less degree of standard error (0.83-0.018) in all characters that confirms homozygosity in these lines.(Rasoazanakolona et al.2017).Molecular analysis , we have use 13 no of SSRs marker of different locus of chromosome to check for homozygosity and uniformity of the 6 DHs line out 13 SSR marker 8 SSR marker has amplified and has shown monomorphic bands. Which confirms that the generated lines were complete DHs lines. These lines were at trail under MLT for there evaluation.(Naik et al.(2016)
Description
RICE DOUBLED HAPLOID PRODUCTION AND HOMOZYGOSITY TESTING USING PHENOTYPIC AND MOLECULAR APPROACHES
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