Allelic Polymorphism of Exon 3 of Leptin Gene in Nilagiri Sheep Identified by Sequencing and Pcr-Rflp
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Date
2014-06
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Abstract
The discovery of hormone leptin had led to better understanding of the energy
balance control. Leptin regulates feed intake, energy metabolism and body composition and
plays a critical role in regulating body weight and growth in mammals. Characterization of
Leptin gene (LEP) would not only help us understand the potential of this gene as marker but
will also help us understand the genetic diversity of indigenous breeds and help in their
conservation. In the present study the polymorphism in Exon 3 of LEP gene in Nilagiri sheep
was done by sequencing and genotyping by PCR-RFLP. Five primers were designed to
amplify the entire Exon 3 of LEP gene. Two polymorphisms 16973 G>A (SNP-L1) and
17476 C>T (SNP-L2) were detected in the Exon 3 in Nilagiri sheep. Animals of SNP-L1
were homozygous to the A allele. On genotyping the SNP-L2 by PCR-RFLP using restriction
enzyme BsrDI, it was found that 77 per cent of the animals were of the CC genotype and 23
animals of the CT genotype. The allelic frequencies of C and T were 0.87 and 0.13
respectively. This shows that the LEP gene is polymorphic in Nilagiri sheep and PCR-RFLP
is an appropriate tool to evaluate genetic variability.
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Veterinary Science, Genetic & Biotechnology