IN VITRO PROPAGATION STUDIES ON STRAWBERRY (FRAGARIA X ANANASSA DUCH.) CV. CHANDLER

Loading...
Thumbnail Image
Date
2009
Journal Title
Journal ISSN
Volume Title
Publisher
Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu
Abstract
The present investigations were carried out at the Tissue Culture Laboratory, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Udheywalla, to study the various aspects of in vitro propagation of strawberry (Fragaria x ananassa Duch.) Cv. Chandler whereas the performance of plantlets produced in vitro and in vivo were evaluated in the Research Orchard of Division of Fruit Science, FOA, Udheywalla, Jammu, during the year 2006-09.The vegetative bud of mature strawberry plant was used as the explant material. Sterilization treatment involving mercuric chloride (0.1 %) for 3 minutes gave minimum contamination with maximum establishment of the vegetative bud explants of strawberry. MS medium supplemented with BAP 1.00 mg/1 and GA<sub>3</sub> 2.00 mg/l was the best for culture establishment whereas maximum proliferation was obtained on MS medium augumented with BAP 2.00 mg/l and GA<sub>3</sub> 2.00 mg/l. In all the three serial subculturing also, this treatment gave best results as far as proliferation was concerned. Lowering down of agar concentration in the medium to 0.4 per cent was found to be more suitable for in vitro rooting and survival of plantlets. MS medium (half strength) supplemented with IBA 1.00 mg/1 and activated charcoal 200 mg/1 gelled with 0.4 per cent agar gave cent per cent rooting in the shoots of strawberry. Out of the pH levels tested, the pH 5.5 was found to be the best for in vitro root and shoot characters of strawberry plantlets. Out of the different carbohydrate sources used, rooting was obtained only on the medium containing sucrose 1 to 3 per cent whereas on other carbohydrate sources, the cultures dried after 3 weeks of incubation. Medium containing sucrose 3 per cent gave cent per cent rooting of shoots and survival of strawberry plantlets. The plantlets transplanted 4 weeks after root initiation and acclimatized by keeping the individual plantlet covered with glass beaker /bottle at 26 ± 2 °C under continuous light intensity of approximately 800 lux resulted in cent per cent survival; followed by 83.33 per cent survival when the plantlets were covered with polythene sheet in groups and kept at 26 ± 2 °C under continuous light intensity of approximately 800 lux. The potting mixture containing soil: sand: FYM (1:1:1 v/v/v) was found to be the most suitable for plantlets. The in vitro regenerated strawberry plantlets showed their superiority over in vivo regenerated plantlets in terms of growth parameters whereas the fruits harvested from in vitro regenerated plants were smaller in size and more acidic recording yield reduction of 12.44 per cent as compared to in vivo regenerated plantlets. The unit cost of plantlet upto polyhouse stage, based on the 1,00,000 plantlets capacity of the production unit was estimated to be Rs. 4.00.
Description
Keywords
null
Citation
No. of references 162
Collections