Regeneration of plantlets through anther culture of maize (Zea mays L.)
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Date
2017
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Birsa Agricultural University, Kanke, Ranchi, Jharkhand
Abstract
Maize (Zea Mays L.) is one of the most important crops around the world
because of its importance in direct and indirect production of food for human
consumption. Because of limited land resources, expanding population, plant
diseases and insect pests stresses, traditional breeding methods have not
consistently met the demand for maize, considering both quality and quantity.
Androgenesis via anther culture is one of the efficient methods for producing
haploid and double haploid plants. In maize, anther culture is an effective
technology for the production of DH population.
In the present study it has been tried to establish an efficient in vitro
method for haploid plant regeneration via anther culture of Zea mays which was
limited by low response rate and high genoytype dependence for haploid
regeneration. For decontamination during surface sterilization of Z. Mays, the
highest recorded survival percentage of explants (variety BM-1, BVM-2 and
Suwan) were achieved by treating the anthers with 0.5% of HgCl2 for five
minutes. Absolutely no response was observed in Anther either in MS and N6
basal media supplemented with different concentrations of hormones. However
only some swelling in the anther was observed in MS medium after 40 days of
inoculations with 2 mg/l 2,4-D and 4 mg/l 2,4-D and 30 days of inoculations for
N6 medium with 1mg/l 2,4-D, 2mg/l 2,4-D, 3mg/l 2,4-D and 2mg/l 2,4-D
+1mg/l Kn. Apart from change in colour in the anthers after 30 days of
inoculation in N6 medium supplemented with charcoal, no further response
were observed.
Description
Regeneration of plantlets through anther culture of maize (Zea mays L.)
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