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STUDIES ON CULTIVATION OF MEDICINAL MUSHROOM REISHI [GANODERMA LUCIDUM (CURT.: FR.) P. KARST.]

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No. of references 108
15
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2011
Raina, P.K.
Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu
110
Mushroom reishi, Microscopic, Biochemical analysis, Ganoderma lucidum, Growth requirement
STUDIES ON CULTIVATION OF MEDICINAL MUSHROOM REISHI [GANODERMA LUCIDUM (CURT.: FR.) P. KARST.]
STUDIES ON CULTIVATION OF MEDICINAL MUSHROOM REISHI [GANODERMA LUCIDUM (CURT.: FR.) P. KARST.]
Ph.D

The present investigation "Studies on cultivation of Medicinal Mushroom Reishi [Ganoderma lucidum (Curt.: Fr.) P. Karst.]" was carried out during 2008-2010. The studies were aimed at generating data on microscopic and macroscopic characteristics, suitable media and physiological growth requirements, fruiting substrate evaluation, optimization of spawn concentration and method of spawning and biochemical analysis of sporophores and fruiting substrates of medicinal mushroom Reishi (G. lucidum). Among different nutrient media tested, the best mycelial growth of G. lucidum was recorded on malt extract agar and malt extract broth, irrespective of strains tested. The temperature and preferential pH levels that initiated maximum mycelial growth were 30-35°C (optimum 30°C) and 5-6 (optimum 6.0), respectively. The best moisture range of the substrate that initiated maximum mycelial growth of G. lucidum was found between 65-70 per cent, however 65 per cent moisture level in the substrate proved to be optimum. While evaluating different spawn concentrations for spawn run, primordial initiation, sporophore maturation and biological efficiency, it was found that higher the spawn concentration, earlier is the spawn run and primordial initiation but for maturation, effect of spawn concentration was non significant. While evaluating spawning method, through spawning proved superior over top layer spawning for early spawn run and maturation of sporophores. The evaluation of different substrates for growth and development of different G. lucidum strains proved sawdust + wheat bran (20%) + calcium carbonate (2%), to be best substrate followed by sugarcane bagasse + wheat bran (20%) + calcium carbonate (2%). The evaluation of different woodchips and sawdust substrates of different tree species for obtaining higher yield proved sawdust + woodchips (1:1) of Dalbergia sissoo tree, to be best substrate for obtaining highest biological efficiency followed by sawdust + woodchips (1:1) of Mangifera indica tree. The estimation of proximate-composition of sundried sporophores of G. lucidum strains revealed significant difference among the strains with regard to dry matter, moisture, crude fibre and total carbohydrate contents, while as total ash, crude fat and crude protein contents showed non-significant variation. The dry matter, moisture, total ash, crude fat, crude protein, crude fibre and total carbohydrate contents ranged between 88.10-90.62 ; 9.37-11.90 ; 8.28-9.26 ; 6.17-7.36 ; 22.30-23.17 ; 2.50-3.90 and 46.15-48.98 per cent, respectively. The estimation of tri-terpenoid content at different developmental stages revealed that tri- terpenoid content during primordial stage remained high and dropped during elongation and maturation stages. The biochemical analysis of the fruiting substrate before and after G. lucidum cultivation suggested that G. lucidum fungus degrades lignin, cellulose and hemi-cellulose but preferentially it degrades lignin more than cellulose and hemi-cellulose. Keywords: Ganoderma lucidum. Growth requirement, Substrate, Biochemical study

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