TOXICO-BIOCHEMICAL AND REPRODUCTIVE ABNORMALITIES INDUCED BY REPEATED ORAL ADMINISTRATION OF METALAXYL IN WISTAR RATS
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Date
2013
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Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu
Abstract
The present study was designed to evaluate haemato-biochemical alterations, oxidative stress parameters, hepatotoxicity and reproductive abnormalities induced by the chronic exposure of metalaxyl fungicide in Wistar rats of both sexes. Rats of 100-200 g body weight (b.wt) were divided into four groups of twenty animals each and were subjected to various daily oral treatment regimes for 98 days. Group I and group II served as control male and female respectively, receiving only distilled water orally, whereas group III and IV received metalaxyl @ 75 mg/kg b.wt daily in distilled water. A significant (P<0.05) decrease in Hb, TEC, TLC, PCV, MCV, MCH, MCHC and neutrophil count was observed in metalaxyl exposed group, whereas, lymphocyte counts and ESR values of were significantly (P<0.05) increased. Total serum proteins, albumin and globulin levels revealed a significant decrease while AST, ALT, ALP, ACP, Serum creatinine, BUN, cholesterol and triglycerides were increased significantly (P<0.05) in the treatment groups as compared to the controls. Metalaxyl caused marked elevation in malondialdehyde and GST whereas, a significant decrease in SOD, glutathione, GPx and catalase activities were observed. A significant decrease was observed in sperm count, viability and motility. Further live pups per litter and pup weights were also decreased significantly in exposed groups. Histopathologically, liver revealed congested central vein with bridging fibrosis and mononuclear cell infiltration. Kidneys also revealed congested glomerular capillaries. Ovaries and uterus showed architectural distortion and atresia of follicles, degeneration and necrosis of endometrium. Testes also showed architectural distortion mainly of developing sperms, inter-epithelial edema in epididymis and seminal vesiculitis represented by leukocytic infiltration.
Keywords: Metalaxyl, Haemato-biochemical, Hepatotoxicity, Oxidative stress Reproductive.
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