STUDIES ON SHIGA TOXIN PRODUCING E. COLI FROM FOODS OF ANIMAL ORIGIN AND CLINICAL CASES
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Date
2011
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Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu
Abstract
A total of 400 samples, 50 each from mutton, chicken meat, milk, milk products, diarrhoeic and non-diarrhoeic cattle stool samples and diarrhoeic and non-diarrhoeic animal handlers stools were processed and 34.25% of samples revealed E. coli. The prevalence of E. coli in different samples was 30%, 40%, 40%, 10%, 64%, 50%, 30% and 10% in mutton, chicken meat, milk, milk products, diarrhoeic cattle, non-diarrhoeic cattle, diarrhoeic animal handlers and non-diarrhoeic animal handlers respectively. The isolated E. coli belonged to forty serogroups on the basis of 'O' antigen, with O60 (9.48%) being predominant followed by O123 (6.56%). The PCR using specific primers for stx1, stx2, genes detected 25.75% STEC with a prevalence of 20% in mutton 30% each in chicken and milk, 8% in milk products, 52% in diarrhoeic cattle and 36% in non-diarrhoeic cattle, 24% in diarrhoeic and 6% in non-diarrhoeic animal handlers. STEC predominated in diarrhoeic cattle over non-diarrhoeic cattle. Prevalence of enteropathogenic E. coli was 4% and 9(2.25%) possessed EHEC-hlyA gene. The isolated EHEC-hlyA gene positive isolates revealed α- haemolysis on sheep blood agar. 47 of 120(39.16%) isolates revealed multidrug resistance to four or more antibiotics. 51% of the isolates were resistant to Amoxycillin, 44.16% to Ampicillin, 40% to Cefotaxime, 19.16% to Amikacin, 6.66% to Chloramphenicol and 13.33% to Gentamicin. The cell free supernatant from serogroups O15, O22, O55, O60, O71, O86, O102, O111, O116 and O139 produced the cytopathic effect on verocells.
Key words: STEC, EPEC, prevalence, serogroups. cattle, animal handlers, Verocells.
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