Callus Mediated in Vitro Regeneration studies in Banana (MUSA SP.) CV. Safed Velchi
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Date
2016-10
Authors
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DBSKKV., Dapoli
Abstract
PLANT BIOTECHNOLOGY CENTRE
COLLEGE OF AGRICULTURE, DAPOLI
DR. BALASAHEB SAWANT KONKAN KRISHI VIDYAPEETH, DAPOLI
Name of Student : Mr. Badhe Ajinkya Sharad
Registration No. : 0016
Name and Designation : Dr. S. V. Sawardekar
of Research Guide Associate Professor
Plant Biotechnology Centre
College of Agriculture, Dapoli, Dist. Ratnagiri
Title of Thesis : Callus Mediated In Vitro Regeneration Studies in Banana (Musa sp.) cv. Safed velchi.
Academic Year : 2014-2016
ABSTRACT
Present investigation aimed, to standardize sterilization technique for in vitro culture of banana (cv. Safed velchi) and to optimize media combinations for callus induction and in vitro regeneration. The study was conducted in Completely Randomized Design at Plant Biotechnology Centre, Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli, Dist. Ratnagiri (M.S.) during the academic year 2014-2016.
In surface sterilization, of Meristem explant, treatment involving Carbandazime (1%) for 30 min, Sodium Hypocloride (5%) for 10 min, Alcohol (70%) for 1 min and Cefotaxime (200mg/l) for 30 min duration recorded maximum aseptic culture establishment (91.66%). In male flower explant, treatment involving Alcohol (70%) for 10 min duration recorded maximum aseptic culture establishment (88.33%). Among leaf, Male flower and Meristem explants, Meristem explant showed significantly better callus proliferation compared to leaf and Male flower explants. In leaf explant MS medium along with 2.0 mg/l 2,4-D + 0.5mg/ l BAP+ 0.5mg/l NAA, observed to be the best combination for callus establishment. On this combination, leaf explant showed 63.33% establishment. For male flower explant MS medium along with 2.5 mg/l 2, 4-D + 0.5mg/ l BAP + 0.5mg/l NAA showed 81.66% callus establishment. Whereas in meristem explant MS medium along with 4 mg/l 2, 4-D + 0.5mg/ l BAP + 0.5 mg/l NAA showed 88.33% callus establishment.
The proliferated callus were cut in to pieces and average 0.5 gm of callus put in to regeneration media. It has been observed that in all regeneration combinations tried, the media combination (MS + 5 mg/l BAP + 1.5mg/l NAA) showed development and shoots. The callus in all other media were greenish but did not showed regeneration.
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